TY - JOUR
T1 - Effect of Calcium Hydroxide on Proinflammatory Cytokines and Neuropeptides
AU - Khan, Asma A.
AU - Sun, Xiaoling
AU - Hargreaves, Kenneth M.
N1 - Funding Information:
This study was funded in part by the AAE Foundation, NIDCR K23 DE14864, P01 DA16719 and R01 NS45186.
PY - 2008/11
Y1 - 2008/11
N2 - Calcium hydroxide, a widely used intracanal medicament, is known to exert an antimicrobial effect and to degrade bacterial-derived lipopolysaccharides. However, little is known about the effect of Ca(OH)2 on endogenous inflammatory mediators such as interleukin-1α (IL-1α), tumor necrosis factor-α (TNF-α), and calcitonin gene-related peptide (CGRP). This is an important gap in knowledge because these inflammatory mediators play an important role in mediating the pathogenesis of periradicular periodontitis. We tested the hypothesis that Ca(OH)2 denatures IL-1α, TNF-α, and CGRP. Human IL-1α (0.125 ng/mL), TNF-α (0.2 ng/mL), and CGRP (0.25 ng/mL) were incubated with Ca(OH)2 (0.035 mg/mL) for 1-7 days. At the end of the incubation period, the pH of the samples was neutralized, and the concentrations of the mediators were measured by immunoassays. Data were analyzed with one-way analysis of variance and Bonferroni multiple comparison tests. The results indicate that Ca(OH)2 denatures IL-1α, TNF-α, and CGRP by 50%-100% during the testing periods (P < .001). We concluded that denaturation of these proinflammatory mediators is a potential mechanism by which Ca(OH)2 contributes to the resolution of periradicular periodontitis.
AB - Calcium hydroxide, a widely used intracanal medicament, is known to exert an antimicrobial effect and to degrade bacterial-derived lipopolysaccharides. However, little is known about the effect of Ca(OH)2 on endogenous inflammatory mediators such as interleukin-1α (IL-1α), tumor necrosis factor-α (TNF-α), and calcitonin gene-related peptide (CGRP). This is an important gap in knowledge because these inflammatory mediators play an important role in mediating the pathogenesis of periradicular periodontitis. We tested the hypothesis that Ca(OH)2 denatures IL-1α, TNF-α, and CGRP. Human IL-1α (0.125 ng/mL), TNF-α (0.2 ng/mL), and CGRP (0.25 ng/mL) were incubated with Ca(OH)2 (0.035 mg/mL) for 1-7 days. At the end of the incubation period, the pH of the samples was neutralized, and the concentrations of the mediators were measured by immunoassays. Data were analyzed with one-way analysis of variance and Bonferroni multiple comparison tests. The results indicate that Ca(OH)2 denatures IL-1α, TNF-α, and CGRP by 50%-100% during the testing periods (P < .001). We concluded that denaturation of these proinflammatory mediators is a potential mechanism by which Ca(OH)2 contributes to the resolution of periradicular periodontitis.
KW - CGRP
KW - Calcium hydroxide
KW - cytokines
KW - interleukin-1
KW - neuropeptides
KW - tumor necrosis factor
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U2 - 10.1016/j.joen.2008.08.020
DO - 10.1016/j.joen.2008.08.020
M3 - Article
C2 - 18928847
AN - SCOPUS:53349168411
SN - 0099-2399
VL - 34
SP - 1360
EP - 1363
JO - Journal of endodontics
JF - Journal of endodontics
IS - 11
ER -