Effect of antimalarial drugs on rat enterocyte mitochondrial phospholipase D activity

Muniswamy Madesh, Kunissery A. Balasubramanian

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We have earlier shown that enterocyte mitochondria contain a phospholipase D (PLD) activity which can be stimulated by oxygen free radicals, divalent cations and polyamines. The functional significance of this enzyme in mitochondria is not known but it can be investigated using selective inhibitors. In the present study, mitochondrial PLD was activated by exposure to oxidants (X+XO or menadione), calcium or polyamines and the effect of antimalarial drugs, chloroquine, amodiaquin and primaquine on PLD activity was studied. Chloroquine and amodiaquine inhibited Ca2+ stimulated PLD activity in dose dependent manner whereas these drugs had no significant effect on PLD activated by oxidants or polyamines. Increasing the calcium concentration relieved the PLD inhibition by these drugs. Primaquine did not have any effect on calcium stimulated PLD activity whereas it slightly activated the enzyme. These results indicate that chloroquine and amodiaquine may bind with calcium making it unavailable for PLD activation.

Original languageEnglish (US)
Pages (from-to)177-184
Number of pages8
JournalLife Sciences
Volume62
Issue number2
DOIs
StatePublished - Dec 5 1997
Externally publishedYes

Keywords

  • Antimalarial drugs
  • Calcium
  • Enterocyte mitochondria
  • Oxidants
  • Phospholipase D
  • Polyamines

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Fingerprint Dive into the research topics of 'Effect of antimalarial drugs on rat enterocyte mitochondrial phospholipase D activity'. Together they form a unique fingerprint.

  • Cite this