TY - JOUR
T1 - Effect of aging and caloric restriction on the mitochondrial proteome
AU - Chang, Jinsook
AU - Cornell, John E.
AU - Van Remmen, Holly
AU - Hakala, Kevin
AU - Ward, Walter F.
AU - Richardson, Arlan
N1 - Funding Information:
ACKNOWLEDGMENTS This work was supported by a Merit Review grant (HVR, AR), an Environmental Hazards Center grant from the Department of Veterans
Funding Information:
Affairs (HVR, AR) and by National Institutes of Health grants RO1 AG025362-01 (WFW) and RO1 AG23843 (AR).
Funding Information:
We thank the University of Texas Health Science Center at San Antonio Institutional Mass Spectrometry Laboratory, supported in part by a San Antonio Cancer Institute grant (CA54174), for assistance in protein identification. CORRESPONDENCE Address correspondence to Walter F. Ward, PhD, Department of Physiology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive-MSC 7756, San Antonio, TX 78229-3900. E-mail: wardw@uthscsa.edu REFERENCES 1. Saraste M. Oxidative phosphorylation at the fin de siecle. Science. 1999;283:1488–1493.
PY - 2007/3
Y1 - 2007/3
N2 - The rat mitochondrial proteome was analyzed using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), and proteins altered by age or caloric restriction (CR) were identified using mass spectrometry. Of 2061 mitochondrial proteins analyzed in the three tissues, a significant change with age occurred in 25 liver proteins (19 increased, 6 decreased), 3 heart proteins (1 increased, 2 decreased), and 5 skeletal muscle proteins (all increased). CR prevented the age-related change in the level of one liver mitochondrial protein, altered the levels of four proteins (one increased, three decreased) from heart, and one protein (decreased) from skeletal muscle. Identification of the proteins that changed with age or CR revealed that they were varied among the three tissues, that is, not one mitochondrial protein was changed, in common, by age or CR in any tissue studied. Thus, the effect of age on the mitochondrial proteome appears to be tissue-specific, and CR has a minor effect on age-related protein changes.
AB - The rat mitochondrial proteome was analyzed using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), and proteins altered by age or caloric restriction (CR) were identified using mass spectrometry. Of 2061 mitochondrial proteins analyzed in the three tissues, a significant change with age occurred in 25 liver proteins (19 increased, 6 decreased), 3 heart proteins (1 increased, 2 decreased), and 5 skeletal muscle proteins (all increased). CR prevented the age-related change in the level of one liver mitochondrial protein, altered the levels of four proteins (one increased, three decreased) from heart, and one protein (decreased) from skeletal muscle. Identification of the proteins that changed with age or CR revealed that they were varied among the three tissues, that is, not one mitochondrial protein was changed, in common, by age or CR in any tissue studied. Thus, the effect of age on the mitochondrial proteome appears to be tissue-specific, and CR has a minor effect on age-related protein changes.
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U2 - 10.1093/gerona/62.3.223
DO - 10.1093/gerona/62.3.223
M3 - Article
C2 - 17389719
AN - SCOPUS:34248556312
VL - 62
SP - 223
EP - 234
JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
SN - 1079-5006
IS - 3
ER -