Effect of age on the function of mitochondria isolated from brain and heart tissue

Y. J.D. Chiu, Arlan Richardson

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

The ADP: O ratios and rates of State 3 and State 4 respiration were measured for mitochondria isolated from heart and brain tissue of 3-32-month-old Fischer F344 rats using the following substrates: succinate, β-hydroxybutyrate, palmitylcarnitine, glutamate, glutamate plus malate, and pyruvate plus malate. No significant age-related change in either the ADP: O ratio or State 4 respiration by isolated heart and brain mitochondria was observed with any of the substrates tested. The rate of State 3 respiration did not change with increasing age using succinate as substrates. However, the rate of State 3 respiration did change significantly as a function of age with isolated heart mitochondria using β-hydroxybutyrate, palmitylcarnitine, glutamate, glutamate plus malate, and pyruvate plus malate and with isolated brain mitochondria using glutamate, glutamate plus malate, and pyruvate plus malate. Between 12 and 32 months of age, the rate of State 3 respiration by isolated heart mitochondria decreased 27-74%.

Original languageEnglish (US)
Pages (from-to)511-517
Number of pages7
JournalExperimental Gerontology
Volume15
Issue number6
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • Biochemistry
  • Aging
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Cell Biology

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