Effect of age on RNA synthesis by rat hepatocytes

William Kreamer, Nora Zorich, Daniel S H Liu, Arlan Richardson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Original languageEnglish
Pages (from-to)27-36
Number of pages10
JournalExperimental Gerontology
Volume14
Issue number1
DOIs
StatePublished - 1979
Externally publishedYes

Fingerprint

Orotic Acid
Uridine Triphosphate
Rats
Hepatocytes
RNA
High pressure liquid chromatography
High Pressure Liquid Chromatography
Acids

ASJC Scopus subject areas

  • Aging
  • Medicine(all)

Cite this

Kreamer, W., Zorich, N., Liu, D. S. H., & Richardson, A. (1979). Effect of age on RNA synthesis by rat hepatocytes. Experimental Gerontology, 14(1), 27-36. https://doi.org/10.1016/0531-5565(79)90005-6

Effect of age on RNA synthesis by rat hepatocytes. / Kreamer, William; Zorich, Nora; Liu, Daniel S H; Richardson, Arlan.

In: Experimental Gerontology, Vol. 14, No. 1, 1979, p. 27-36.

Research output: Contribution to journalArticle

Kreamer, W, Zorich, N, Liu, DSH & Richardson, A 1979, 'Effect of age on RNA synthesis by rat hepatocytes', Experimental Gerontology, vol. 14, no. 1, pp. 27-36. https://doi.org/10.1016/0531-5565(79)90005-6
Kreamer, William ; Zorich, Nora ; Liu, Daniel S H ; Richardson, Arlan. / Effect of age on RNA synthesis by rat hepatocytes. In: Experimental Gerontology. 1979 ; Vol. 14, No. 1. pp. 27-36.
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title = "Effect of age on RNA synthesis by rat hepatocytes",
abstract = "The incorporation of (3H) orotic acid into RNA by hepatocytes from rats of various ages was studied. A 37{\%} decrease in the incorporation of (3H) orotic acid into RNA was observed by hepatocytes isolated from 30-month-old rats compared to 12-month-old rats. There was no age-related difference in the incorporation of (3H) orotic acid into acid-insoluble material by hepatocytes. The incorporation of (3H) orotic acid into UTP by hepatocytes was determined by high pressure liquid chromatography. The (3H) orotic acid was rapidly converted to UTP by hepatocytes, and the specific activity of the UTP reached a steady state after 10 min of incubation. There was no significant difference in the specific activity of the UTP in hepatocytes isolated from rats ranging in age from 6 to 25 months. Therefore, the age-related decrease in the incorporation of (3H) orotic acid into RNA by hepatocytes is not due to an age-related difference in the uptake or conversion of (3H) orotic acid into UTP.",
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AU - Kreamer, William

AU - Zorich, Nora

AU - Liu, Daniel S H

AU - Richardson, Arlan

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N2 - The incorporation of (3H) orotic acid into RNA by hepatocytes from rats of various ages was studied. A 37% decrease in the incorporation of (3H) orotic acid into RNA was observed by hepatocytes isolated from 30-month-old rats compared to 12-month-old rats. There was no age-related difference in the incorporation of (3H) orotic acid into acid-insoluble material by hepatocytes. The incorporation of (3H) orotic acid into UTP by hepatocytes was determined by high pressure liquid chromatography. The (3H) orotic acid was rapidly converted to UTP by hepatocytes, and the specific activity of the UTP reached a steady state after 10 min of incubation. There was no significant difference in the specific activity of the UTP in hepatocytes isolated from rats ranging in age from 6 to 25 months. Therefore, the age-related decrease in the incorporation of (3H) orotic acid into RNA by hepatocytes is not due to an age-related difference in the uptake or conversion of (3H) orotic acid into UTP.

AB - The incorporation of (3H) orotic acid into RNA by hepatocytes from rats of various ages was studied. A 37% decrease in the incorporation of (3H) orotic acid into RNA was observed by hepatocytes isolated from 30-month-old rats compared to 12-month-old rats. There was no age-related difference in the incorporation of (3H) orotic acid into acid-insoluble material by hepatocytes. The incorporation of (3H) orotic acid into UTP by hepatocytes was determined by high pressure liquid chromatography. The (3H) orotic acid was rapidly converted to UTP by hepatocytes, and the specific activity of the UTP reached a steady state after 10 min of incubation. There was no significant difference in the specific activity of the UTP in hepatocytes isolated from rats ranging in age from 6 to 25 months. Therefore, the age-related decrease in the incorporation of (3H) orotic acid into RNA by hepatocytes is not due to an age-related difference in the uptake or conversion of (3H) orotic acid into UTP.

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