Effect of age on RNA synthesis by rat hepatocytes

William Kreamer, Nora Zorich, Daniel S.H. Liu, Arlan Richardson

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The incorporation of (3H) orotic acid into RNA by hepatocytes from rats of various ages was studied. A 37% decrease in the incorporation of (3H) orotic acid into RNA was observed by hepatocytes isolated from 30-month-old rats compared to 12-month-old rats. There was no age-related difference in the incorporation of (3H) orotic acid into acid-insoluble material by hepatocytes. The incorporation of (3H) orotic acid into UTP by hepatocytes was determined by high pressure liquid chromatography. The (3H) orotic acid was rapidly converted to UTP by hepatocytes, and the specific activity of the UTP reached a steady state after 10 min of incubation. There was no significant difference in the specific activity of the UTP in hepatocytes isolated from rats ranging in age from 6 to 25 months. Therefore, the age-related decrease in the incorporation of (3H) orotic acid into RNA by hepatocytes is not due to an age-related difference in the uptake or conversion of (3H) orotic acid into UTP.

Original languageEnglish (US)
Pages (from-to)27-36
Number of pages10
JournalExperimental Gerontology
Volume14
Issue number1
DOIs
StatePublished - 1979

ASJC Scopus subject areas

  • Biochemistry
  • Aging
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Cell Biology

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