Effect of ACTH on biosynthesis of aldosterone and corticosterone by monolayer cultures of rat adrenal zona glomerulosa cells

Peter J. Hornsby; Michael J. O'Hare; A. Munro Neville

doi:10.1016/0006-291X(73)91163-7

Effect of ACTH on biosynthesis of aldosterone and corticosterone by monolayer cultures of rat adrenal zona glomerulosa cells

Peter J. Hornsby, Michael J. O'Hare, A. Munro Neville

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A method is described for preparing monolayer cultures of zona glomerulosa cells isolated from the rat adrenal cortex. Aldosterone and corticosterone were secreted by the cultures when maintained with medium containing 11 mM K⁺. ACTH, while stimulating aldosterone biosynthesis at first, did not maintain its long-term secretion, yet caused corticosterone production to rise to a steadily maintained level. The significance of this effect is discussed.

Original language	English (US)
Pages (from-to)	1554-1559
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	54
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(73)91163-7
State	Published - Oct 15 1973
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(73)91163-7

Cite this

@article{9d8e4c607e4b462b84211e27ae3a64c9,

title = "Effect of ACTH on biosynthesis of aldosterone and corticosterone by monolayer cultures of rat adrenal zona glomerulosa cells",

abstract = "A method is described for preparing monolayer cultures of zona glomerulosa cells isolated from the rat adrenal cortex. Aldosterone and corticosterone were secreted by the cultures when maintained with medium containing 11 mM K+. ACTH, while stimulating aldosterone biosynthesis at first, did not maintain its long-term secretion, yet caused corticosterone production to rise to a steadily maintained level. The significance of this effect is discussed.",

author = "Hornsby, {Peter J.} and O'Hare, {Michael J.} and {Munro Neville}, A.",

note = "Funding Information: Acknowledgements This work was supported by the Medical Great Britain (Grant no. 970/656/B). 0 ne of us (P.J.H.)wxld Prof. E. J.Ambrose for advice and encouragement, and the Royal London, for a Studentship. We thank the National Institute and Digestive Diseases anti-aldosterone antiserum.",

year = "1973",

month = oct,

day = "15",

doi = "10.1016/0006-291X(73)91163-7",

language = "English (US)",

volume = "54",

pages = "1554--1559",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "4",

}

TY - JOUR

T1 - Effect of ACTH on biosynthesis of aldosterone and corticosterone by monolayer cultures of rat adrenal zona glomerulosa cells

AU - Hornsby, Peter J.

AU - O'Hare, Michael J.

AU - Munro Neville, A.

N1 - Funding Information: Acknowledgements This work was supported by the Medical Great Britain (Grant no. 970/656/B). 0 ne of us (P.J.H.)wxld Prof. E. J.Ambrose for advice and encouragement, and the Royal London, for a Studentship. We thank the National Institute and Digestive Diseases anti-aldosterone antiserum.

PY - 1973/10/15

Y1 - 1973/10/15

N2 - A method is described for preparing monolayer cultures of zona glomerulosa cells isolated from the rat adrenal cortex. Aldosterone and corticosterone were secreted by the cultures when maintained with medium containing 11 mM K+. ACTH, while stimulating aldosterone biosynthesis at first, did not maintain its long-term secretion, yet caused corticosterone production to rise to a steadily maintained level. The significance of this effect is discussed.

AB - A method is described for preparing monolayer cultures of zona glomerulosa cells isolated from the rat adrenal cortex. Aldosterone and corticosterone were secreted by the cultures when maintained with medium containing 11 mM K+. ACTH, while stimulating aldosterone biosynthesis at first, did not maintain its long-term secretion, yet caused corticosterone production to rise to a steadily maintained level. The significance of this effect is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0015799758&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015799758&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(73)91163-7

DO - 10.1016/0006-291X(73)91163-7

M3 - Article

C2 - 4356825

AN - SCOPUS:0015799758

SN - 0006-291X

VL - 54

SP - 1554

EP - 1559

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Effect of ACTH on biosynthesis of aldosterone and corticosterone by monolayer cultures of rat adrenal zona glomerulosa cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this