Effect of a GnRH analogue on rat granulosa cell lactate production in vitro

H. Billig, C. S.S. Rani, C. Ekholm

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The effect of a GnRH analogue ((D-Ala6,des-Gly10-NH2)-GnRH-ethylamide,GnRH) on granulosa and cumulus cell glycolysis in presence or absence of FSH was studied. Cumulus complexes and granulosa cells from PMSG-treated rats were cultured in Eagle's minimal essential medium (MEM) for a period of 72 h. Media were changed at 24 and 48 h and lactate content was assayed by fluorimetry. GnRHa alone stimulated lactate production in granulosa cells. GnRH combined with FSH increased lactate production in granulosa cells during the 0-24 h period and decreased it during the 48-72 h period as compared to FSH alone. GnRHa did not stimulate lactate production in cumulus complexes during 72 h culture in MEM, while FSH did. In a less complex culture medium, BMOC, GnRHa caused a small increase in lactate production and slightly enhanced the FSH effect. In conclusion, GnRHa has a direct stimulatory effect on granulosa cell glycolysis. GnRHa also modulates the FSH stimulation of granulosa cells biphasically, i.e. early enhancement (0-24 h) and late inhibition (48-72 h). GnRHa has no consistent direct effects on cumulus cell glycolysis.

Original languageEnglish (US)
Pages (from-to)112-118
Number of pages7
JournalActa Endocrinologica
Volume105
Issue number1
StatePublished - Jan 1 1984
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology

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