TY - JOUR
T1 - Effect of a GnRH analogue on rat granulosa cell lactate production in vitro
AU - Billig, H.
AU - Rani, C. S.S.
AU - Ekholm, C.
PY - 1984
Y1 - 1984
N2 - The effect of a GnRH analogue ((D-Ala6,des-Gly10-NH2)-GnRH-ethylamide,GnRH) on granulosa and cumulus cell glycolysis in presence or absence of FSH was studied. Cumulus complexes and granulosa cells from PMSG-treated rats were cultured in Eagle's minimal essential medium (MEM) for a period of 72 h. Media were changed at 24 and 48 h and lactate content was assayed by fluorimetry. GnRHa alone stimulated lactate production in granulosa cells. GnRH combined with FSH increased lactate production in granulosa cells during the 0-24 h period and decreased it during the 48-72 h period as compared to FSH alone. GnRHa did not stimulate lactate production in cumulus complexes during 72 h culture in MEM, while FSH did. In a less complex culture medium, BMOC, GnRHa caused a small increase in lactate production and slightly enhanced the FSH effect. In conclusion, GnRHa has a direct stimulatory effect on granulosa cell glycolysis. GnRHa also modulates the FSH stimulation of granulosa cells biphasically, i.e. early enhancement (0-24 h) and late inhibition (48-72 h). GnRHa has no consistent direct effects on cumulus cell glycolysis.
AB - The effect of a GnRH analogue ((D-Ala6,des-Gly10-NH2)-GnRH-ethylamide,GnRH) on granulosa and cumulus cell glycolysis in presence or absence of FSH was studied. Cumulus complexes and granulosa cells from PMSG-treated rats were cultured in Eagle's minimal essential medium (MEM) for a period of 72 h. Media were changed at 24 and 48 h and lactate content was assayed by fluorimetry. GnRHa alone stimulated lactate production in granulosa cells. GnRH combined with FSH increased lactate production in granulosa cells during the 0-24 h period and decreased it during the 48-72 h period as compared to FSH alone. GnRHa did not stimulate lactate production in cumulus complexes during 72 h culture in MEM, while FSH did. In a less complex culture medium, BMOC, GnRHa caused a small increase in lactate production and slightly enhanced the FSH effect. In conclusion, GnRHa has a direct stimulatory effect on granulosa cell glycolysis. GnRHa also modulates the FSH stimulation of granulosa cells biphasically, i.e. early enhancement (0-24 h) and late inhibition (48-72 h). GnRHa has no consistent direct effects on cumulus cell glycolysis.
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U2 - 10.1530/acta.0.1050112
DO - 10.1530/acta.0.1050112
M3 - Article
C2 - 6421039
AN - SCOPUS:0021352578
VL - 105
SP - 112
EP - 118
JO - European Journal of Endocrinology
JF - European Journal of Endocrinology
SN - 0804-4643
IS - 1
ER -