TY - JOUR
T1 - Effect of 20α-dihydroxyprogesterone on the estrogen-induced bioactive luteinizing hormone surge in ovariectomized monkeys
AU - Schenken, R. S.
AU - Williams, R. F.
AU - Hodgen, G. D.
N1 - Funding Information:
Received March 19, 1990; revised and accepted July 30, 1990. *Supported in part by grant P30-10202 from the National Institutes of Health, Bethesda, Maryland. t Reprint requests: Robert S. Schenken, M.D., Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas 78284. :j: Department of Obstetrics and Gynecology, The Jones Institute for Reproductive Medicine.
PY - 1990
Y1 - 1990
N2 - Progestins facilitate estrogen induction of the preovulatory luteinizing hormone (LH) surge. A biologically weaker progestin, 20α-dihydroxyprogesterone, is reportedly increased at midcycle, but the importance of 20α-dihydroxyprogesterone in regulating LH release has not been systematically studied. We therefore evaluated the effect of 2 doses of 20α-dihydroxyprogesterone on the bioactive LH surge onset in ovariectomized monkeys. Chronically cannulated monkeys (n = 10) received estradiol (E2) benzoate (50 μg/kg) with and without subsequent 20α-dihydroxyprogesterone injections (50 μg/kg, n = 3 and 100 μg/kg, n = 3). Blood was collected every 15 minutes over preselected intervals and the plasma assayed for bioactive LH and gonadal steroids. Low-dose 20α-dihydroxyprogesterone administration resulted in periovulatory peripheral 20α-dihydroxyprogesterone levels and did not advance the bioactive LH surge onset. In contrast, high-dose 20α-dihydroxyprogesterone resulted in supraphysiological 20α-dihydroxyprogesterone levels, slight increases in progesterone levels, and significant advancement of the surge over E2 benzoate treatment alone. We conclude that periovulatory levels of 20α-dihydroxyprogesterone do not play a role in modulating the estrogen-induced bioactive LH surge.
AB - Progestins facilitate estrogen induction of the preovulatory luteinizing hormone (LH) surge. A biologically weaker progestin, 20α-dihydroxyprogesterone, is reportedly increased at midcycle, but the importance of 20α-dihydroxyprogesterone in regulating LH release has not been systematically studied. We therefore evaluated the effect of 2 doses of 20α-dihydroxyprogesterone on the bioactive LH surge onset in ovariectomized monkeys. Chronically cannulated monkeys (n = 10) received estradiol (E2) benzoate (50 μg/kg) with and without subsequent 20α-dihydroxyprogesterone injections (50 μg/kg, n = 3 and 100 μg/kg, n = 3). Blood was collected every 15 minutes over preselected intervals and the plasma assayed for bioactive LH and gonadal steroids. Low-dose 20α-dihydroxyprogesterone administration resulted in periovulatory peripheral 20α-dihydroxyprogesterone levels and did not advance the bioactive LH surge onset. In contrast, high-dose 20α-dihydroxyprogesterone resulted in supraphysiological 20α-dihydroxyprogesterone levels, slight increases in progesterone levels, and significant advancement of the surge over E2 benzoate treatment alone. We conclude that periovulatory levels of 20α-dihydroxyprogesterone do not play a role in modulating the estrogen-induced bioactive LH surge.
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U2 - 10.1016/s0015-0282(16)54019-9
DO - 10.1016/s0015-0282(16)54019-9
M3 - Article
C2 - 2245841
AN - SCOPUS:0025609201
SN - 0015-0282
VL - 54
SP - 1146
EP - 1149
JO - Fertility and sterility
JF - Fertility and sterility
IS - 6
ER -