Effect of 20α-dihydroxyprogesterone on the estrogen-induced bioactive luteinizing hormone surge in ovariectomized monkeys

R. S. Schenken, R. F. Williams, G. D. Hodgen

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Progestins facilitate estrogen induction of the preovulatory luteinizing hormone (LH) surge. A biologically weaker progestin, 20α-dihydroxyprogesterone, is reportedly increased at midcycle, but the importance of 20α-dihydroxyprogesterone in regulating LH release has not been systematically studied. We therefore evaluated the effect of 2 doses of 20α-dihydroxyprogesterone on the bioactive LH surge onset in ovariectomized monkeys. Chronically cannulated monkeys (n = 10) received estradiol (E2) benzoate (50 μg/kg) with and without subsequent 20α-dihydroxyprogesterone injections (50 μg/kg, n = 3 and 100 μg/kg, n = 3). Blood was collected every 15 minutes over preselected intervals and the plasma assayed for bioactive LH and gonadal steroids. Low-dose 20α-dihydroxyprogesterone administration resulted in periovulatory peripheral 20α-dihydroxyprogesterone levels and did not advance the bioactive LH surge onset. In contrast, high-dose 20α-dihydroxyprogesterone resulted in supraphysiological 20α-dihydroxyprogesterone levels, slight increases in progesterone levels, and significant advancement of the surge over E2 benzoate treatment alone. We conclude that periovulatory levels of 20α-dihydroxyprogesterone do not play a role in modulating the estrogen-induced bioactive LH surge.

Original languageEnglish (US)
Pages (from-to)1146-1149
Number of pages4
JournalFertility and sterility
Volume54
Issue number6
DOIs
StatePublished - 1990

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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