Ectopic BAT mUCP-1 overexpression in SKM by delivering a BMP7/PRDM16/PGC-1a gene cocktail or single PRMD16 using non-viral UTMD gene therapy

Shuyuan Chen, Raul A. Bastarrachea, Jin Song Shen, Antonio Laviada-Nagel, Ernesto Rodriguez-Ayala, Edna J. Nava-Gonzalez, Pintong Huang, Ralph A. DeFronzo, Jack W. Kent, Paul A. Grayburn

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Here we present our progress in inducing an ectopic brown adipose tissue (BAT) phenotype in skeletal muscle (SKM) as a potential gene therapy for obesity and its comorbidities. We used ultrasound-targeted microbubble destruction (UTMD), a novel targeted, non-viral approach to gene therapy, to deliver genes in the BAT differentiation pathway into rodent SKM to engineer a thermogenic BAT phenotype with ectopic mUCP-1 overexpression. In parallel, we performed a second protocol using wild-type Ucp-1-null knockout mice to test whether the effects of the gene therapy are UCP-1 dependent. Our main findings were a robust cellular presence of mUCP-1 immunostaining (IHC), significantly higher expression levels of mUCP-1 measured by qRT-PCR, and highest temperature elevation measured by infrared thermography in the treated thigh, achieved in rats after delivering the UTMD-PRDM16/PGC-1a/BMP7/hyPB gene cocktail. Interestingly, the weight loss obtained in the treated rats with the triple gene delivery, never recovered the levels observed in the controls in spite of food intake recovery. Our results establish the feasibility of minimally invasive UTMD gene-based therapy administration in SKM, to induce overexpression of ectopic mUCP-1 after delivery of the thermogenic BAT gene program, and describe systemic effects of this intervention on food intake, weight loss, and thermogenesis.

Original languageEnglish (US)
Pages (from-to)497-509
Number of pages13
JournalGene Therapy
Volume25
Issue number7
DOIs
StatePublished - Oct 1 2018

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

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