Early histopathologic events to evolution of colon cancer in C57BL/6 and CF1 mice treated with 1,2-dimethylhydrazine

Michael J Wargovich, A. Medline, W. R. Bruce

Research output: Contribution to journalArticle

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Abstract

After administration of the intestinal carcinogen 1,2-dimethylhydrazine (DMH), C57BL/6J and CF1 mice were observed for early precursor lesions to large bowel cancer. Among the initial events seen following DMH treatment, an abrupt reduction in colonic DNA synthesis was the earliest lesion detectable. The frequency of abberrant colonic nuclei rose shortly after DMH treatment, reaching a maximum value 24 hours later and remaining elevated for 3 days following the exposure. Mucin changes, detected histochemically, and cell kinetic alterations in crypt proliferation rates were observed much later and were a constant feature for both strains following 4 weekly treatments with DMH, while carcinomas appeared in all animals 32 weeks after the start of DMH treatment. The quantitative comparison of these histopathologic observations for the early detection of colon cancer suggests that the induction of colonic nuclear aberrations in the mucosa of the large bowel might provide a sensitive and rapid indication of genotoxicity to this organ and thus might provide the basis for a screening methodology for colon carcinogens.

Original languageEnglish (US)
Pages (from-to)125-131
Number of pages7
JournalJournal of the National Cancer Institute
Volume71
Issue number1
StatePublished - 1983
Externally publishedYes

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1,2-Dimethylhydrazine
Colonic Neoplasms
Carcinogens
Mucins
Early Detection of Cancer
Colon
Mucous Membrane
Carcinoma
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Early histopathologic events to evolution of colon cancer in C57BL/6 and CF1 mice treated with 1,2-dimethylhydrazine. / Wargovich, Michael J; Medline, A.; Bruce, W. R.

In: Journal of the National Cancer Institute, Vol. 71, No. 1, 1983, p. 125-131.

Research output: Contribution to journalArticle

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