Early endothelial damage detected by circulating particles in baboons fed a diet high in simple carbohydrates in conjunction with saturated or unsaturated fat

Qiang Shi, Vida Hodara, Vida Hodara, V. Saroja Voruganti, Karen Rice, Joel E. Michalek, Anthony G. Comuzzie, John L. Vandeberg

Research output: Contribution to journalArticle

4 Scopus citations


Studies have shown that high-fat diets cause blood vessel damage, however, assessing pathological effects accurately and efficiently is difficult. In this study, we measured particle levels of static endothelium (CD31+ and CD105+) and activated endothelium (CD62E+, CD54+ and CD106+) in plasma. We determined individual responses to two dietary regimens in two groups of baboons. One group (n = 10), was fed a diet high in simple carbohydrates and saturated fats (the HSF diet) and the other (n = 8) received a diet high in simple carbohydrates and unsaturated fats (the HUF diet). Plasma samples were collected at 0, 3, and 7 weeks. The percentages of CD31+ and CD62E+ particles were elevated at 3 weeks in animals fed either diet, but these elevations were statistically significant only in animals fed the HUF diet. Surprisingly, both percentages and counts of CD31+ particles were significantly lower at week 7 compared to week 0 and 3 in the HSF group. The median absolute counts of CD105+ particles were progressively elevated over time in the HSF group with a significant increase from week 0 to 7; the pattern was somewhat different for the HUF group with significant increase from week 3 to 7. The counts of CD54+ particles exhibited wide variation in both groups during the dietary challenge, while the median counts of CD106+ particles were significantly lower at week 3 than at week 0 and week 7. Endothelial particles exhibited time-dependent changes, suggesting they were behaving as quantifiable surrogates for the early detection of vascular damage caused by dietary factors.

Original languageEnglish (US)
Pages (from-to)123-132
Number of pages10
JournalAmerican Journal of Cardiovascular Disease
Issue number3
Publication statusPublished - Jan 1 2014



  • Biomarkers
  • Circulating endothelial particles
  • Dietary challenge
  • Nonhuman primate model
  • Vascular damage

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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