Dynamics of the two heterochromatin types during imprinted X chromosome inactivation in vole Microtus levis

Evgeniya A. Vaskova, Elena V. Dementyeva, Alexander I. Shevchenko, Sophia V. Pavlova, Elena V. Grigor'eva, Antonina I. Zhelezova, John L. VandeBerg, Suren M. Zakian

    Research output: Contribution to journalArticlepeer-review

    7 Scopus citations

    Abstract

    In rodent female mammals, there are two forms of X-inactivation - imprinted and random which take place in extraembryonic and embryonic tissues, respectively. The inactive X-chromosome during random X-inactivation was shown to contain two types of facultative heterochromatin that alternate and do not overlap. However, chromatin structure of the inactive X-chromosome during imprinted X-inactivation, especially at early stages, is still not well understood. In this work, we studied chromatin modifications associated with the inactive X-chromosome at different stages of imprinted X-inactivation in a rodent, Microtus levis. It has been found that imprinted X-inactivation in vole occurs in a species-specific manner in two steps. The inactive X-chromosome at early stages of imprinted X-inactivation is characterized by accumulation of H3K9me3, HP1, H4K20me3, and uH2A, resembling to some extent the pattern of repressive chromatin modifications of meiotic sex chromatin. Later, the inactive X-chromosome recruits trimethylated H3K27 and acquires the two types of heterochromatin associated with random X-inactivation.

    Original languageEnglish (US)
    Article numbere88256
    JournalPloS one
    Volume9
    Issue number2
    DOIs
    StatePublished - Feb 4 2014

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)
    • General

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