Dynamics of replication-independent histone turnover in budding yeast

Michael F. Dion, Tommy Kaplan, Minkyu Kim, Stephen Buratowski, Nir Friedman, Oliver J. Rando

Research output: Contribution to journalArticlepeer-review

447 Scopus citations

Abstract

Chromatin plays roles in processes governed by different time scales. To assay the dynamic behavior of chromatin in living cells, we used genomic tiling arrays to measure histone H3 turnover in G1-arrested Saccharomyces cerevisiae at single-nucleosome resolution over 4% of the genome, and at lower (∼265 base pair) resolution over the entire genome. We find that nucleosomes at promoters are replaced more rapidly than at coding regions and that replacement rates over coding regions correlate with polymerase density. In addition, rapid histone turnover is found at known chromatin boundary elements. These results suggest that rapid histone turnover serves to functionally separate chromatin domains and prevent spread of histone states.

Original languageEnglish (US)
Pages (from-to)1405-1408
Number of pages4
JournalScience
Volume315
Issue number5817
DOIs
StatePublished - Mar 9 2007
Externally publishedYes

ASJC Scopus subject areas

  • General

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