Dynamic MCP-1 mRNA response of human aortic endothelial cells (HAEC) to step changes in laminar shear stress

J. B. Guild, A. B. Chromiak, Eugene A Sprague, R. M. Nerem

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Our aim is to study the hemodynamic alteration of monocyte recruitment and adhesion in model blood vessel systems, focusing on the hemodynamic effects of the recruitment factor monocyte chemotactic protein one, MCP-1, gene expression in HAEC. Preconditioning with low shear stress, LS, elevates MCP-1 gene activity and eliminates any transient peak in activity upon switching to high shear stress, HS, without affecting HS-induced gene down-regulation. These data suggest an interaction between the pathways mediating the long term response to LS and the transient cellular response to HS onset.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Place of PublicationPiscataway, NJ, United States
PublisherIEEE
Pages4
Number of pages1
Volume1
ISBN (Print)0780356756
StatePublished - 1999
Externally publishedYes
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

Fingerprint

Endothelial cells
Hemodynamics
Shear stress
Monocyte Chemoattractant Proteins
Genes
Messenger RNA
Blood vessels
Gene expression
Adhesion
Proteins

ASJC Scopus subject areas

  • Bioengineering

Cite this

Guild, J. B., Chromiak, A. B., Sprague, E. A., & Nerem, R. M. (1999). Dynamic MCP-1 mRNA response of human aortic endothelial cells (HAEC) to step changes in laminar shear stress. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 1, pp. 4). Piscataway, NJ, United States: IEEE.

Dynamic MCP-1 mRNA response of human aortic endothelial cells (HAEC) to step changes in laminar shear stress. / Guild, J. B.; Chromiak, A. B.; Sprague, Eugene A; Nerem, R. M.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 Piscataway, NJ, United States : IEEE, 1999. p. 4.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Guild, JB, Chromiak, AB, Sprague, EA & Nerem, RM 1999, Dynamic MCP-1 mRNA response of human aortic endothelial cells (HAEC) to step changes in laminar shear stress. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 1, IEEE, Piscataway, NJ, United States, pp. 4, Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS), Atlanta, GA, USA, 10/13/99.
Guild JB, Chromiak AB, Sprague EA, Nerem RM. Dynamic MCP-1 mRNA response of human aortic endothelial cells (HAEC) to step changes in laminar shear stress. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1. Piscataway, NJ, United States: IEEE. 1999. p. 4
Guild, J. B. ; Chromiak, A. B. ; Sprague, Eugene A ; Nerem, R. M. / Dynamic MCP-1 mRNA response of human aortic endothelial cells (HAEC) to step changes in laminar shear stress. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 Piscataway, NJ, United States : IEEE, 1999. pp. 4
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