Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in store-operated calcium influx: Evidence for similarities in store-operated and calcium release-activated calcium channel components

Ling Ong Hwei, Tai Cheng Kwong, Xibao Liu, Bidhan C. Bandyopadhyay, Biman C. Paria, Jonathan Soboloff, Biswaranjan Pani, Yousang Gwack, Sonal Srikanth, Brij B Singh, Donald Gill, Indu S. Ambudkar

Research output: Contribution to journalArticle

302 Citations (Scopus)

Abstract

Store-operated calcium entry (SOCE) is a ubiquitous mechanism that is mediated by distinct SOC channels, ranging from the highly selective calcium release-activated Ca2+ (CRAC) channel in rat basophilic leukemia and other hematopoietic cells to relatively Ca2+-selective or non-selective SOC channels in other cells. Although the exact composition of these channels is not yet established, TRPC1 contributes to SOC channels and regulation of physiological function of a variety of cell types. Recently, Orai1 and STIM1 have been suggested to be sufficient for generating CRAC channels. Here we show that Orai1 and STIM1 are also required for TRPC1-SOC channels. Knockdown of TRPC1, Orai1, or STIM1 attenuated, whereas overexpression of TRPC1, but not Orai1 or STIM1, induced an increase in SOC entry and ISOC in human salivary gland cells. All three proteins were co-localized in the plasma membrane region of cells, and thapsigargin increased co-immunoprecipitation of TRPC1 with STIM1, and Orai1 in human salivary gland cells as well as dispersed mouse submandibular gland cells. In aggregate, the data presented here reveal that all three proteins are essential for generation of ISOC in these cells and that dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in activation of SOC channel in response to internal Ca2+ store depletion. Thus, these data suggest a common molecular basis for SOC and CRAC channels.

Original languageEnglish (US)
Pages (from-to)9105-9116
Number of pages12
JournalJournal of Biological Chemistry
Volume282
Issue number12
DOIs
StatePublished - Mar 23 2007
Externally publishedYes

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Calcium Channels
Calcium
Thapsigargin
Cell membranes
Salivary Glands
Rats
Proteins
Chemical activation
Calcium Release Activated Calcium Channels
Submandibular Gland
Chemical analysis
Immunoprecipitation
Leukemia
Cell Membrane

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in store-operated calcium influx : Evidence for similarities in store-operated and calcium release-activated calcium channel components. / Hwei, Ling Ong; Kwong, Tai Cheng; Liu, Xibao; Bandyopadhyay, Bidhan C.; Paria, Biman C.; Soboloff, Jonathan; Pani, Biswaranjan; Gwack, Yousang; Srikanth, Sonal; Singh, Brij B; Gill, Donald; Ambudkar, Indu S.

In: Journal of Biological Chemistry, Vol. 282, No. 12, 23.03.2007, p. 9105-9116.

Research output: Contribution to journalArticle

Hwei, Ling Ong ; Kwong, Tai Cheng ; Liu, Xibao ; Bandyopadhyay, Bidhan C. ; Paria, Biman C. ; Soboloff, Jonathan ; Pani, Biswaranjan ; Gwack, Yousang ; Srikanth, Sonal ; Singh, Brij B ; Gill, Donald ; Ambudkar, Indu S. / Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in store-operated calcium influx : Evidence for similarities in store-operated and calcium release-activated calcium channel components. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 12. pp. 9105-9116.
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abstract = "Store-operated calcium entry (SOCE) is a ubiquitous mechanism that is mediated by distinct SOC channels, ranging from the highly selective calcium release-activated Ca2+ (CRAC) channel in rat basophilic leukemia and other hematopoietic cells to relatively Ca2+-selective or non-selective SOC channels in other cells. Although the exact composition of these channels is not yet established, TRPC1 contributes to SOC channels and regulation of physiological function of a variety of cell types. Recently, Orai1 and STIM1 have been suggested to be sufficient for generating CRAC channels. Here we show that Orai1 and STIM1 are also required for TRPC1-SOC channels. Knockdown of TRPC1, Orai1, or STIM1 attenuated, whereas overexpression of TRPC1, but not Orai1 or STIM1, induced an increase in SOC entry and ISOC in human salivary gland cells. All three proteins were co-localized in the plasma membrane region of cells, and thapsigargin increased co-immunoprecipitation of TRPC1 with STIM1, and Orai1 in human salivary gland cells as well as dispersed mouse submandibular gland cells. In aggregate, the data presented here reveal that all three proteins are essential for generation of ISOC in these cells and that dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in activation of SOC channel in response to internal Ca2+ store depletion. Thus, these data suggest a common molecular basis for SOC and CRAC channels.",
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T1 - Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in store-operated calcium influx

T2 - Evidence for similarities in store-operated and calcium release-activated calcium channel components

AU - Hwei, Ling Ong

AU - Kwong, Tai Cheng

AU - Liu, Xibao

AU - Bandyopadhyay, Bidhan C.

AU - Paria, Biman C.

AU - Soboloff, Jonathan

AU - Pani, Biswaranjan

AU - Gwack, Yousang

AU - Srikanth, Sonal

AU - Singh, Brij B

AU - Gill, Donald

AU - Ambudkar, Indu S.

PY - 2007/3/23

Y1 - 2007/3/23

N2 - Store-operated calcium entry (SOCE) is a ubiquitous mechanism that is mediated by distinct SOC channels, ranging from the highly selective calcium release-activated Ca2+ (CRAC) channel in rat basophilic leukemia and other hematopoietic cells to relatively Ca2+-selective or non-selective SOC channels in other cells. Although the exact composition of these channels is not yet established, TRPC1 contributes to SOC channels and regulation of physiological function of a variety of cell types. Recently, Orai1 and STIM1 have been suggested to be sufficient for generating CRAC channels. Here we show that Orai1 and STIM1 are also required for TRPC1-SOC channels. Knockdown of TRPC1, Orai1, or STIM1 attenuated, whereas overexpression of TRPC1, but not Orai1 or STIM1, induced an increase in SOC entry and ISOC in human salivary gland cells. All three proteins were co-localized in the plasma membrane region of cells, and thapsigargin increased co-immunoprecipitation of TRPC1 with STIM1, and Orai1 in human salivary gland cells as well as dispersed mouse submandibular gland cells. In aggregate, the data presented here reveal that all three proteins are essential for generation of ISOC in these cells and that dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in activation of SOC channel in response to internal Ca2+ store depletion. Thus, these data suggest a common molecular basis for SOC and CRAC channels.

AB - Store-operated calcium entry (SOCE) is a ubiquitous mechanism that is mediated by distinct SOC channels, ranging from the highly selective calcium release-activated Ca2+ (CRAC) channel in rat basophilic leukemia and other hematopoietic cells to relatively Ca2+-selective or non-selective SOC channels in other cells. Although the exact composition of these channels is not yet established, TRPC1 contributes to SOC channels and regulation of physiological function of a variety of cell types. Recently, Orai1 and STIM1 have been suggested to be sufficient for generating CRAC channels. Here we show that Orai1 and STIM1 are also required for TRPC1-SOC channels. Knockdown of TRPC1, Orai1, or STIM1 attenuated, whereas overexpression of TRPC1, but not Orai1 or STIM1, induced an increase in SOC entry and ISOC in human salivary gland cells. All three proteins were co-localized in the plasma membrane region of cells, and thapsigargin increased co-immunoprecipitation of TRPC1 with STIM1, and Orai1 in human salivary gland cells as well as dispersed mouse submandibular gland cells. In aggregate, the data presented here reveal that all three proteins are essential for generation of ISOC in these cells and that dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in activation of SOC channel in response to internal Ca2+ store depletion. Thus, these data suggest a common molecular basis for SOC and CRAC channels.

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