Dual reporter approaches for identification of Cre efficacy and astrocyte heterogeneity

Joachim Degen, Pavel Dublin, Jiong Zhang, Radoslaw Dobrowolski, Melanie Jokwitz, Khalad Karram, Jacqueline Trotter, Ronald Jabs, Klaus Willecke, Christian Steinhäuser, Martin Theis

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Gene inactivation reporters are powerful tools to circumvent limitations of the widely used Cre/loxP system of conditional mutagenesis. With new conditional transgenic mouse lines expressing the enhanced cyan fluorescent protein (ECFP) instead of connexin43 (Cx43) after Cre-mediated recombination, we demonstrate dual reporter approaches to simultaneously examine astrocyte subpopulations expressing different connexins, identify compensatory up-regulation within gene families, and quantify Cre-mediated deletion at the allelic level. Analysis of a newly generated Cx43 knock-in ECFP mouse revealed an unexpected heterogeneity of Cx43-expressing astrocytes across brain areas.

Original languageEnglish (US)
Pages (from-to)4576-4583
Number of pages8
JournalFASEB Journal
Volume26
Issue number11
DOIs
StatePublished - Nov 1 2012

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Keywords

  • Conditional knock-in
  • Connexin
  • GFAP
  • Gap junctions

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Degen, J., Dublin, P., Zhang, J., Dobrowolski, R., Jokwitz, M., Karram, K., Trotter, J., Jabs, R., Willecke, K., Steinhäuser, C., & Theis, M. (2012). Dual reporter approaches for identification of Cre efficacy and astrocyte heterogeneity. FASEB Journal, 26(11), 4576-4583. https://doi.org/10.1096/fj.12-207183