Dual reporter approaches for identification of Cre efficacy and astrocyte heterogeneity

Joachim Degen; Pavel Dublin; Jiong Zhang; Radoslaw Dobrowolski; Melanie Jokwitz; Khalad Karram; Jacqueline Trotter; Ronald Jabs; Klaus Willecke; Christian Steinhäuser; Martin Theis

doi:10.1096/fj.12-207183

Dual reporter approaches for identification of Cre efficacy and astrocyte heterogeneity

Joachim Degen, Pavel Dublin, Jiong Zhang, Radoslaw Dobrowolski, Melanie Jokwitz, Khalad Karram, Jacqueline Trotter, Ronald Jabs, Klaus Willecke, Christian Steinhäuser, Martin Theis

Department of Cell Systems & Anatomy

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Gene inactivation reporters are powerful tools to circumvent limitations of the widely used Cre/loxP system of conditional mutagenesis. With new conditional transgenic mouse lines expressing the enhanced cyan fluorescent protein (ECFP) instead of connexin43 (Cx43) after Cre-mediated recombination, we demonstrate dual reporter approaches to simultaneously examine astrocyte subpopulations expressing different connexins, identify compensatory up-regulation within gene families, and quantify Cre-mediated deletion at the allelic level. Analysis of a newly generated Cx43 knock-in ECFP mouse revealed an unexpected heterogeneity of Cx43-expressing astrocytes across brain areas.

Original language	English (US)
Pages (from-to)	4576-4583
Number of pages	8
Journal	FASEB Journal
Volume	26
Issue number	11
DOIs	https://doi.org/10.1096/fj.12-207183
State	Published - Nov 2012

Keywords

Conditional knock-in
Connexin
GFAP
Gap junctions

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Genetics

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10.1096/fj.12-207183

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abstract = "Gene inactivation reporters are powerful tools to circumvent limitations of the widely used Cre/loxP system of conditional mutagenesis. With new conditional transgenic mouse lines expressing the enhanced cyan fluorescent protein (ECFP) instead of connexin43 (Cx43) after Cre-mediated recombination, we demonstrate dual reporter approaches to simultaneously examine astrocyte subpopulations expressing different connexins, identify compensatory up-regulation within gene families, and quantify Cre-mediated deletion at the allelic level. Analysis of a newly generated Cx43 knock-in ECFP mouse revealed an unexpected heterogeneity of Cx43-expressing astrocytes across brain areas.",

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AU - Degen, Joachim

AU - Dublin, Pavel

AU - Zhang, Jiong

AU - Dobrowolski, Radoslaw

AU - Jokwitz, Melanie

AU - Karram, Khalad

AU - Trotter, Jacqueline

AU - Jabs, Ronald

AU - Willecke, Klaus

AU - Steinhäuser, Christian

AU - Theis, Martin

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AB - Gene inactivation reporters are powerful tools to circumvent limitations of the widely used Cre/loxP system of conditional mutagenesis. With new conditional transgenic mouse lines expressing the enhanced cyan fluorescent protein (ECFP) instead of connexin43 (Cx43) after Cre-mediated recombination, we demonstrate dual reporter approaches to simultaneously examine astrocyte subpopulations expressing different connexins, identify compensatory up-regulation within gene families, and quantify Cre-mediated deletion at the allelic level. Analysis of a newly generated Cx43 knock-in ECFP mouse revealed an unexpected heterogeneity of Cx43-expressing astrocytes across brain areas.

KW - Conditional knock-in

KW - Connexin

KW - GFAP

KW - Gap junctions

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Dual reporter approaches for identification of Cre efficacy and astrocyte heterogeneity

Abstract

Keywords

ASJC Scopus subject areas

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