TY - JOUR
T1 - Dual radiolabeled liposomes
T2 - Biodistribution studies and localization of focal sites of infection in rats
AU - Awasthi, V. D.
AU - Goins, Beth
AU - Klipper, Robert
AU - Phillips, William T.
N1 - Funding Information:
The authors acknowledge the help of Dr. Alan Rudolph and Richard Cliff at the Naval Research Laboratory, Washington, DC, for determining the size of the liposomes. This study was funded by a grant from the National Institutes of Health (ROI HL 53052).
PY - 1998/2
Y1 - 1998/2
N2 - Liposomes encapsulating both glutathione and deferoxamine were labeled with 99mTc-HMPAO and 111In-oxine at the same time. These dual radiolabeled liposomes were intravenously injected in rats with S. aureus infection in thigh. The target-to-background ratio (T/BG) increased from 2.9 at 2 h to 4.4 at 8 h in 99mTc images. In 111In images, T/BG of 5.5 at 8 h increased to 10.5 by 48 h. The 24-h spleen uptake of 111In- and 99mTc-liposomes was 24.14%ID and 8.91%ID. In femur, 99mTc-liposomes remained at ~10.5%ID, but 111In-liposomes increased from ~11%ID at 4 h to ~25.5%ID at 24 h. The simultaneous presence of 99mTc and 111In in the liposomes resulted in good early (2-8 h) as well as delayed (24-48 h) images delineating the infection site.
AB - Liposomes encapsulating both glutathione and deferoxamine were labeled with 99mTc-HMPAO and 111In-oxine at the same time. These dual radiolabeled liposomes were intravenously injected in rats with S. aureus infection in thigh. The target-to-background ratio (T/BG) increased from 2.9 at 2 h to 4.4 at 8 h in 99mTc images. In 111In images, T/BG of 5.5 at 8 h increased to 10.5 by 48 h. The 24-h spleen uptake of 111In- and 99mTc-liposomes was 24.14%ID and 8.91%ID. In femur, 99mTc-liposomes remained at ~10.5%ID, but 111In-liposomes increased from ~11%ID at 4 h to ~25.5%ID at 24 h. The simultaneous presence of 99mTc and 111In in the liposomes resulted in good early (2-8 h) as well as delayed (24-48 h) images delineating the infection site.
KW - Dual radiolabeled liposomes
KW - In-liposomes
KW - Infection imaging
KW - Long circulating liposomes
KW - Tc-liposomes
UR - http://www.scopus.com/inward/record.url?scp=0031983684&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031983684&partnerID=8YFLogxK
U2 - 10.1016/S0969-8051(97)00162-5
DO - 10.1016/S0969-8051(97)00162-5
M3 - Article
C2 - 9468030
AN - SCOPUS:0031983684
VL - 25
SP - 155
EP - 160
JO - International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology
JF - International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology
SN - 0969-8051
IS - 2
ER -