Drug-loaded biodegradable microspheres for image-guided combinatory epigenetic therapy in cells

Ronald X. Xu; Jeff S. Xu; Tao Zuo; Rulong Shen; Tim H. Huang; Michael F. Tweedle

doi:10.1117/1.3548878

Drug-loaded biodegradable microspheres for image-guided combinatory epigenetic therapy in cells

Ronald X. Xu, Jeff S. Xu, Tao Zuo, Rulong Shen, Tim H. Huang, Michael F. Tweedle

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We synthesize drug-loaded poly (lactic-co-glycolic acid) (PLGA) microspheres for image-guided combinatory epigenetic therapy in MCF-10A human mammary epithelial cells. LY294002 and Nile Red are encapsulated in microspheres for sustained drug release and fluorescence microscopic imaging. Drug-loaded microspheres target MCF-10A cells through a three-step binding process involving biotinylated antibody, streptavidin, and biotinylated microspheres. LY294002 loaded microspheres and 5-Aza-2-deoxycytidine are applied to MCF-10A cells for combinatory PI3K/AKT inhibition and deoxyribonucleic acid (DNA) demethylation. Our study implies the technical potential of disease targeting and image-guided combinatory epigenetic therapy using drug-loaded multifunctional biodegradable PLGA microspheres.

Original language	English (US)
Article number	020507
Journal	Journal of biomedical optics
Volume	16
Issue number	2
DOIs	https://doi.org/10.1117/1.3548878
State	Published - Feb 2011
Externally published	Yes

Keywords

Biotinylation
DNA demethylation
Epigenetic therapy
Fluorescence imaging
Microsphere
PI3K/AKT inhibition
Poly (lactic-co-glycolic acid) (PLGA)

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomedical Engineering
Biomaterials

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title = "Drug-loaded biodegradable microspheres for image-guided combinatory epigenetic therapy in cells",

abstract = "We synthesize drug-loaded poly (lactic-co-glycolic acid) (PLGA) microspheres for image-guided combinatory epigenetic therapy in MCF-10A human mammary epithelial cells. LY294002 and Nile Red are encapsulated in microspheres for sustained drug release and fluorescence microscopic imaging. Drug-loaded microspheres target MCF-10A cells through a three-step binding process involving biotinylated antibody, streptavidin, and biotinylated microspheres. LY294002 loaded microspheres and 5-Aza-2-deoxycytidine are applied to MCF-10A cells for combinatory PI3K/AKT inhibition and deoxyribonucleic acid (DNA) demethylation. Our study implies the technical potential of disease targeting and image-guided combinatory epigenetic therapy using drug-loaded multifunctional biodegradable PLGA microspheres.",

keywords = "Biotinylation, DNA demethylation, Epigenetic therapy, Fluorescence imaging, Microsphere, PI3K/AKT inhibition, Poly (lactic-co-glycolic acid) (PLGA)",

author = "Xu, {Ronald X.} and Xu, {Jeff S.} and Tao Zuo and Rulong Shen and Huang, {Tim H.} and Tweedle, {Michael F.}",

note = "Funding Information: This work was supported in part by National Institutes of Health (U54 CA113001 to T.H.H., R01 CA 069065 to T.H.H.) Department of Defense Breast Cancer Research Program (W81XWH-07 to R.X.X.), and OSU Department of Radiology (startup fund to M.F.T.). The authors are grateful to Dr. Haiming Ding of Department of Radiology for his technical input and Mr. Jiwei Huang of Department of Biomedical Engineering for his image processing assistance.",

year = "2011",

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doi = "10.1117/1.3548878",

language = "English (US)",

volume = "16",

journal = "Journal of biomedical optics",

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AU - Xu, Ronald X.

AU - Xu, Jeff S.

AU - Zuo, Tao

AU - Shen, Rulong

AU - Huang, Tim H.

AU - Tweedle, Michael F.

N1 - Funding Information: This work was supported in part by National Institutes of Health (U54 CA113001 to T.H.H., R01 CA 069065 to T.H.H.) Department of Defense Breast Cancer Research Program (W81XWH-07 to R.X.X.), and OSU Department of Radiology (startup fund to M.F.T.). The authors are grateful to Dr. Haiming Ding of Department of Radiology for his technical input and Mr. Jiwei Huang of Department of Biomedical Engineering for his image processing assistance.

PY - 2011/2

Y1 - 2011/2

N2 - We synthesize drug-loaded poly (lactic-co-glycolic acid) (PLGA) microspheres for image-guided combinatory epigenetic therapy in MCF-10A human mammary epithelial cells. LY294002 and Nile Red are encapsulated in microspheres for sustained drug release and fluorescence microscopic imaging. Drug-loaded microspheres target MCF-10A cells through a three-step binding process involving biotinylated antibody, streptavidin, and biotinylated microspheres. LY294002 loaded microspheres and 5-Aza-2-deoxycytidine are applied to MCF-10A cells for combinatory PI3K/AKT inhibition and deoxyribonucleic acid (DNA) demethylation. Our study implies the technical potential of disease targeting and image-guided combinatory epigenetic therapy using drug-loaded multifunctional biodegradable PLGA microspheres.

AB - We synthesize drug-loaded poly (lactic-co-glycolic acid) (PLGA) microspheres for image-guided combinatory epigenetic therapy in MCF-10A human mammary epithelial cells. LY294002 and Nile Red are encapsulated in microspheres for sustained drug release and fluorescence microscopic imaging. Drug-loaded microspheres target MCF-10A cells through a three-step binding process involving biotinylated antibody, streptavidin, and biotinylated microspheres. LY294002 loaded microspheres and 5-Aza-2-deoxycytidine are applied to MCF-10A cells for combinatory PI3K/AKT inhibition and deoxyribonucleic acid (DNA) demethylation. Our study implies the technical potential of disease targeting and image-guided combinatory epigenetic therapy using drug-loaded multifunctional biodegradable PLGA microspheres.

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KW - DNA demethylation

KW - Epigenetic therapy

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Drug-loaded biodegradable microspheres for image-guided combinatory epigenetic therapy in cells

Abstract

Keywords

ASJC Scopus subject areas

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