Drug-loaded biodegradable microspheres for image-guided combinatory epigenetic therapy in cells

Ronald X. Xu, Jeff S. Xu, Tao Zuo, Rulong Shen, Hui-ming Huang, Michael F. Tweedle

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We synthesize drug-loaded poly (lactic-co-glycolic acid) (PLGA) microspheres for image-guided combinatory epigenetic therapy in MCF-10A human mammary epithelial cells. LY294002 and Nile Red are encapsulated in microspheres for sustained drug release and fluorescence microscopic imaging. Drug-loaded microspheres target MCF-10A cells through a three-step binding process involving biotinylated antibody, streptavidin, and biotinylated microspheres. LY294002 loaded microspheres and 5-Aza-2-deoxycytidine are applied to MCF-10A cells for combinatory PI3K/AKT inhibition and deoxyribonucleic acid (DNA) demethylation. Our study implies the technical potential of disease targeting and image-guided combinatory epigenetic therapy using drug-loaded multifunctional biodegradable PLGA microspheres.

Original languageEnglish (US)
Article number020507
JournalJournal of Biomedical Optics
Volume16
Issue number2
DOIs
StatePublished - Feb 2011
Externally publishedYes

Fingerprint

Microspheres
therapy
drugs
acids
cells
Pharmaceutical Preparations
decitabine
chemotherapy
antibodies
deoxyribonucleic acid
Drug therapy
fluorescence
Streptavidin
Acids
Phosphatidylinositol 3-Kinases
Antibodies
DNA
Fluorescence
Imaging techniques

Keywords

  • Biotinylation
  • DNA demethylation
  • Epigenetic therapy
  • Fluorescence imaging
  • Microsphere
  • PI3K/AKT inhibition
  • Poly (lactic-co-glycolic acid) (PLGA)

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Drug-loaded biodegradable microspheres for image-guided combinatory epigenetic therapy in cells. / Xu, Ronald X.; Xu, Jeff S.; Zuo, Tao; Shen, Rulong; Huang, Hui-ming; Tweedle, Michael F.

In: Journal of Biomedical Optics, Vol. 16, No. 2, 020507, 02.2011.

Research output: Contribution to journalArticle

Xu, Ronald X. ; Xu, Jeff S. ; Zuo, Tao ; Shen, Rulong ; Huang, Hui-ming ; Tweedle, Michael F. / Drug-loaded biodegradable microspheres for image-guided combinatory epigenetic therapy in cells. In: Journal of Biomedical Optics. 2011 ; Vol. 16, No. 2.
@article{508a4226f0864471b52d5c0f82be1fb1,
title = "Drug-loaded biodegradable microspheres for image-guided combinatory epigenetic therapy in cells",
abstract = "We synthesize drug-loaded poly (lactic-co-glycolic acid) (PLGA) microspheres for image-guided combinatory epigenetic therapy in MCF-10A human mammary epithelial cells. LY294002 and Nile Red are encapsulated in microspheres for sustained drug release and fluorescence microscopic imaging. Drug-loaded microspheres target MCF-10A cells through a three-step binding process involving biotinylated antibody, streptavidin, and biotinylated microspheres. LY294002 loaded microspheres and 5-Aza-2-deoxycytidine are applied to MCF-10A cells for combinatory PI3K/AKT inhibition and deoxyribonucleic acid (DNA) demethylation. Our study implies the technical potential of disease targeting and image-guided combinatory epigenetic therapy using drug-loaded multifunctional biodegradable PLGA microspheres.",
keywords = "Biotinylation, DNA demethylation, Epigenetic therapy, Fluorescence imaging, Microsphere, PI3K/AKT inhibition, Poly (lactic-co-glycolic acid) (PLGA)",
author = "Xu, {Ronald X.} and Xu, {Jeff S.} and Tao Zuo and Rulong Shen and Hui-ming Huang and Tweedle, {Michael F.}",
year = "2011",
month = "2",
doi = "10.1117/1.3548878",
language = "English (US)",
volume = "16",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "2",

}

TY - JOUR

T1 - Drug-loaded biodegradable microspheres for image-guided combinatory epigenetic therapy in cells

AU - Xu, Ronald X.

AU - Xu, Jeff S.

AU - Zuo, Tao

AU - Shen, Rulong

AU - Huang, Hui-ming

AU - Tweedle, Michael F.

PY - 2011/2

Y1 - 2011/2

N2 - We synthesize drug-loaded poly (lactic-co-glycolic acid) (PLGA) microspheres for image-guided combinatory epigenetic therapy in MCF-10A human mammary epithelial cells. LY294002 and Nile Red are encapsulated in microspheres for sustained drug release and fluorescence microscopic imaging. Drug-loaded microspheres target MCF-10A cells through a three-step binding process involving biotinylated antibody, streptavidin, and biotinylated microspheres. LY294002 loaded microspheres and 5-Aza-2-deoxycytidine are applied to MCF-10A cells for combinatory PI3K/AKT inhibition and deoxyribonucleic acid (DNA) demethylation. Our study implies the technical potential of disease targeting and image-guided combinatory epigenetic therapy using drug-loaded multifunctional biodegradable PLGA microspheres.

AB - We synthesize drug-loaded poly (lactic-co-glycolic acid) (PLGA) microspheres for image-guided combinatory epigenetic therapy in MCF-10A human mammary epithelial cells. LY294002 and Nile Red are encapsulated in microspheres for sustained drug release and fluorescence microscopic imaging. Drug-loaded microspheres target MCF-10A cells through a three-step binding process involving biotinylated antibody, streptavidin, and biotinylated microspheres. LY294002 loaded microspheres and 5-Aza-2-deoxycytidine are applied to MCF-10A cells for combinatory PI3K/AKT inhibition and deoxyribonucleic acid (DNA) demethylation. Our study implies the technical potential of disease targeting and image-guided combinatory epigenetic therapy using drug-loaded multifunctional biodegradable PLGA microspheres.

KW - Biotinylation

KW - DNA demethylation

KW - Epigenetic therapy

KW - Fluorescence imaging

KW - Microsphere

KW - PI3K/AKT inhibition

KW - Poly (lactic-co-glycolic acid) (PLGA)

UR - http://www.scopus.com/inward/record.url?scp=79957869737&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79957869737&partnerID=8YFLogxK

U2 - 10.1117/1.3548878

DO - 10.1117/1.3548878

M3 - Article

VL - 16

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 2

M1 - 020507

ER -