Drug-loaded biodegradable microspheres for image-guided combinatory epigenetic therapy in cells

Ronald X. Xu, Jeff S. Xu, Tao Zuo, Rulong Shen, Tim H. Huang, Michael F. Tweedle

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

We synthesize drug-loaded poly (lactic-co-glycolic acid) (PLGA) microspheres for image-guided combinatory epigenetic therapy in MCF-10A human mammary epithelial cells. LY294002 and Nile Red are encapsulated in microspheres for sustained drug release and fluorescence microscopic imaging. Drug-loaded microspheres target MCF-10A cells through a three-step binding process involving biotinylated antibody, streptavidin, and biotinylated microspheres. LY294002 loaded microspheres and 5-Aza-2-deoxycytidine are applied to MCF-10A cells for combinatory PI3K/AKT inhibition and deoxyribonucleic acid (DNA) demethylation. Our study implies the technical potential of disease targeting and image-guided combinatory epigenetic therapy using drug-loaded multifunctional biodegradable PLGA microspheres.

Original languageEnglish (US)
Article number020507
JournalJournal of Biomedical Optics
Volume16
Issue number2
DOIs
StatePublished - Feb 1 2011

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Keywords

  • Biotinylation
  • DNA demethylation
  • Epigenetic therapy
  • Fluorescence imaging
  • Microsphere
  • PI3K/AKT inhibition
  • Poly (lactic-co-glycolic acid) (PLGA)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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