Drosophila Ringmaker regulates microtubule stabilization and axonal extension during embryonic development

Rosa E. Mino, Stephen L. Rogers, April L Risinger, Cristina Rohena, Swati Banerjee, Manzoor Bhat

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Axonal growth and targeting are fundamental to the organization of the nervous system, and require active engagement of the cytoskeleton. Polymerization and stabilization of axonal microtubules is central to axonal growth and maturation of neuronal connectivity. Studies have suggested that members of the tubulin polymerization promoting protein (TPPP, also known as P25α) family are involved in cellular process extension. However, no in vivo knockout data exists regarding its role in axonal growth during development. Here, we report the characterization of Ringmaker (Ringer; CG45057), the only Drosophila homolog of long p25a proteins. Immunohistochemical analyses indicate that Ringer expression is dynamically regulated in the embryonic central nervous system (CNS). ringer-null mutants show cell misplacement, and errors in axonal extension and targeting. Ultrastructural examination of ringer mutants revealed defective microtubule morphology and organization. Primary neuronal cultures of ringer mutants exhibit defective axonal extension, and Ringer expression in cells induced microtubule stabilization and bundling into rings. In vitro assays showed that Ringer directly affects tubulin, and promotes microtubule bundling and polymerization. Together, our studies uncover an essential function of Ringer in axonal extension and targeting through proper microtubule organization.

Original languageEnglish (US)
Pages (from-to)3282-3294
Number of pages13
JournalJournal of Cell Science
Volume129
Issue number17
DOIs
StatePublished - 2016

Fingerprint

Microtubules
Drosophila
Embryonic Development
Polymerization
Tubulin
Growth
Cytoskeleton
Growth and Development
Nervous System
Proteins
Central Nervous System

Keywords

  • Axon growth
  • Cytoskeleton
  • Microtubules
  • Nervous system
  • TPPP
  • Tubulin polymerization
  • Tubulin stabilization

ASJC Scopus subject areas

  • Cell Biology

Cite this

Drosophila Ringmaker regulates microtubule stabilization and axonal extension during embryonic development. / Mino, Rosa E.; Rogers, Stephen L.; Risinger, April L; Rohena, Cristina; Banerjee, Swati; Bhat, Manzoor.

In: Journal of Cell Science, Vol. 129, No. 17, 2016, p. 3282-3294.

Research output: Contribution to journalArticle

Mino, Rosa E. ; Rogers, Stephen L. ; Risinger, April L ; Rohena, Cristina ; Banerjee, Swati ; Bhat, Manzoor. / Drosophila Ringmaker regulates microtubule stabilization and axonal extension during embryonic development. In: Journal of Cell Science. 2016 ; Vol. 129, No. 17. pp. 3282-3294.
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