Drosophila contactin, a homolog of vertebrate contactin, is required for septate junction organization and paracellular barrier function

Catherine Faivre-Sarrailh, Swati Banerjee, Jingjun Li, Michael Hortsch, Monique Laval, Manzoor Bhat

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Septate junctions (SJs) in epithelial and neuronal cells play an important role in the formation and maintenance of charge and size selective barriers. They form the basis for the ensheathment of nerve fibers in Drosophila and for the attachment of myelin loops to axonal surface in vertebrates. The cell-adhesion molecules NRX IV/Caspr/Paranodin (NCP1), contactin and Neurofascin-155 (NF-155) are all present at the vertebrate axo-glial SJs. Mutational analyses have shown that vertebrate NCP1 and its Drosophila homolog, Neurexin IV (NRX IV) are required for the formation of SJs. In this study, we report the genetic, molecular and biochemical characterization of the Drosophila homolog of vertebrate contactin, CONT. Ultrastructural and dye-exclusion analyses of Cont mutant embryos show that CONT is required for organization of SJs and paracellular barrier function. We show that CONT, Neuroglian (NRG) (Drosophila homolog of NF-155) and NRX IV are interdependent for their SJ localization and these proteins form a tripartite complex. Hence, our data provide evidence that the organization of SJs is dependent on the interactions between these highly conserved cell-adhesion molecules.

Original languageEnglish (US)
Pages (from-to)4931-4942
Number of pages12
JournalDevelopment
Volume131
Issue number20
DOIs
StatePublished - Oct 2004
Externally publishedYes

Fingerprint

Contactins
Drosophila
Vertebrates
Cell Adhesion Molecules
Molecular Biology
Myelin Sheath
Nerve Fibers
Neuroglia
Coloring Agents
Embryonic Structures
Epithelial Cells
Maintenance
Proteins

Keywords

  • Axo-glial interactions
  • F3/Contactin
  • Neurexin IV
  • Neuroglian
  • Septate junctions

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Drosophila contactin, a homolog of vertebrate contactin, is required for septate junction organization and paracellular barrier function. / Faivre-Sarrailh, Catherine; Banerjee, Swati; Li, Jingjun; Hortsch, Michael; Laval, Monique; Bhat, Manzoor.

In: Development, Vol. 131, No. 20, 10.2004, p. 4931-4942.

Research output: Contribution to journalArticle

Faivre-Sarrailh, Catherine ; Banerjee, Swati ; Li, Jingjun ; Hortsch, Michael ; Laval, Monique ; Bhat, Manzoor. / Drosophila contactin, a homolog of vertebrate contactin, is required for septate junction organization and paracellular barrier function. In: Development. 2004 ; Vol. 131, No. 20. pp. 4931-4942.
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