TY - JOUR
T1 - Drosophila APC2 and Armadillo participate in tethering mitotic spindles to cortical actin
AU - McCartney, Brooke M.
AU - McEwen, Donald G.
AU - Grevengoed, Elizabeth
AU - Maddox, Paul
AU - Bejsovec, Amy
AU - Peifer, Mark
N1 - Funding Information:
ACKNOWLEDGMENTS We thank E. Schejter, T. Uemura, M. Takeichi, E. Siegfried and the Bloomington Drosophila Stock Center for fly stocks and antibodies, T. Salmon, K. Bloom and the three reviewers for helpful suggestions, and S. Whitfield for assistance with the figures. This work was supported by grants to M.P. from the Human Frontiers Science Program and the NIH (GM47857), and to A.B. from the NSF IBN97-34072. B.M. was supported by NIH NRSA 1F32CA79172, D.M. was supported by NIH NRSA 1F32GM19824, E.G. was supported by NIH 5T32CA71341, and M.P. was supported in part by a Career Development Award from the U.S. Army Breast Cancer Research Program. Correspondence and requests for materials should be addressed to M.P.
PY - 2001
Y1 - 2001
N2 - Proper positioning of mitotic spindles ensures equal allocation of chromosomes to daughter cells. This often involves interactions between spindle and astral microtubules and cortical actin1. In yeast and Caenorhabditis elegans, some of the protein machinery that connects spindles and cortex has been identified but, in most animal cells, this process remains mysterious. Here, we report that the tumour suppresser homologue APC2 and its binding partner Armadillo both play roles in spindle anchoring during the syncytial mitoses of early Drosophila embryos. Armadillo, α-catenin and APC2 all localize to sites of cortical spindle attachment. APC2-Armadillo complexes often localize with interphase microtubules. Zeste-white 3 kinase, which can phosphorylate Armadillo and APC, is also crucial for spindle positioning and regulates the localization of APC2-Armadillo complexes. Together, these data suggest that APC2, Armadillo and α-catenin provide an important link between spindles and cortical actin, and that this link is regulated by Zeste-white 3 kinase.
AB - Proper positioning of mitotic spindles ensures equal allocation of chromosomes to daughter cells. This often involves interactions between spindle and astral microtubules and cortical actin1. In yeast and Caenorhabditis elegans, some of the protein machinery that connects spindles and cortex has been identified but, in most animal cells, this process remains mysterious. Here, we report that the tumour suppresser homologue APC2 and its binding partner Armadillo both play roles in spindle anchoring during the syncytial mitoses of early Drosophila embryos. Armadillo, α-catenin and APC2 all localize to sites of cortical spindle attachment. APC2-Armadillo complexes often localize with interphase microtubules. Zeste-white 3 kinase, which can phosphorylate Armadillo and APC, is also crucial for spindle positioning and regulates the localization of APC2-Armadillo complexes. Together, these data suggest that APC2, Armadillo and α-catenin provide an important link between spindles and cortical actin, and that this link is regulated by Zeste-white 3 kinase.
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U2 - 10.1038/ncb1001-933
DO - 10.1038/ncb1001-933
M3 - Article
C2 - 11584277
AN - SCOPUS:0034789121
VL - 3
SP - 933
EP - 938
JO - Nature Cell Biology
JF - Nature Cell Biology
SN - 1465-7392
IS - 10
ER -