Drosophila APC2 and Armadillo participate in tethering mitotic spindles to cortical actin

Brooke M. McCartney, Donald G. McEwen, Elizabeth Grevengoed, Paul Maddox, Amy Bejsovec, Mark Peifer

Research output: Contribution to journalArticlepeer-review

140 Scopus citations

Abstract

Proper positioning of mitotic spindles ensures equal allocation of chromosomes to daughter cells. This often involves interactions between spindle and astral microtubules and cortical actin1. In yeast and Caenorhabditis elegans, some of the protein machinery that connects spindles and cortex has been identified but, in most animal cells, this process remains mysterious. Here, we report that the tumour suppresser homologue APC2 and its binding partner Armadillo both play roles in spindle anchoring during the syncytial mitoses of early Drosophila embryos. Armadillo, α-catenin and APC2 all localize to sites of cortical spindle attachment. APC2-Armadillo complexes often localize with interphase microtubules. Zeste-white 3 kinase, which can phosphorylate Armadillo and APC, is also crucial for spindle positioning and regulates the localization of APC2-Armadillo complexes. Together, these data suggest that APC2, Armadillo and α-catenin provide an important link between spindles and cortical actin, and that this link is regulated by Zeste-white 3 kinase.

Original languageEnglish (US)
Pages (from-to)933-938
Number of pages6
JournalNature Cell Biology
Volume3
Issue number10
DOIs
StatePublished - Oct 22 2001
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Drosophila APC2 and Armadillo participate in tethering mitotic spindles to cortical actin'. Together they form a unique fingerprint.

Cite this