T cell lines with a novel phenotype (CD3+ TCR-α/β+ CD4- CD8-) were developed from the peripheral blood of a patient with a combined immunodeficiency and tissue injury resembling graft-vs-host disease. One of these IL-2-dependent T cell lines demonstrated non-MHC-restricted cytolytic function against tumor targets, syngeneic and allogeneic fibroblasts, and PHA blasts from allogeneic donors. The other cell line only became cytotoxic in the presence of lectin or anti-CD3 antibody. The two cell lines also differed in their expression of the T-200 gene products CD45RO (gp180) and CD45RA (gp220). Both cell lines produced tumor necrosis factor-α and -β and IFN-γ activity when activated with mitogens or PMA and IL-1. The in vitro functions of these T-cell lines suggest a potential role for α/β double-negative T lymphocytes in tissue injury resembling graft-vs-host disease.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Immunology|
|State||Published - 1990|
ASJC Scopus subject areas
- Immunology and Allergy