Double-negative (CD4- CD8-) T cells with an α/β T cell receptor. Non-MHC-restricted cytolytic activity and lymphokine production

E. G. Brooks, D. P. Wirt, R. M. Goldblum, S. Vaidya, M. T. Asuncion, J. C. Patterson, C. F. Ware, G. R. Klimpel

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

T cell lines with a novel phenotype (CD3+ TCR-α/β+ CD4- CD8-) were developed from the peripheral blood of a patient with a combined immunodeficiency and tissue injury resembling graft-vs-host disease. One of these IL-2-dependent T cell lines demonstrated non-MHC-restricted cytolytic function against tumor targets, syngeneic and allogeneic fibroblasts, and PHA blasts from allogeneic donors. The other cell line only became cytotoxic in the presence of lectin or anti-CD3 antibody. The two cell lines also differed in their expression of the T-200 gene products CD45RO (gp180) and CD45RA (gp220). Both cell lines produced tumor necrosis factor-α and -β and IFN-γ activity when activated with mitogens or PMA and IL-1. The in vitro functions of these T-cell lines suggest a potential role for α/β double-negative T lymphocytes in tissue injury resembling graft-vs-host disease.

Original languageEnglish (US)
Pages (from-to)4507-4512
Number of pages6
JournalJournal of Immunology
Volume144
Issue number12
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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