Double-negative (CD4- CD8-) T cells with an α/β T cell receptor: Non-MHC-restricted cytolytic activity and lymphokine production

Edward G Brooks, Daniel P. Wirt, Randall M. Goldblum, Smita Vaidya, Maria T. Asuncion, James C. Patterson, Carl F. Ware, Gary R. Klimpel

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

T cell lines with a novel phenotype (CD3+ TCR-α/β+ CD4- CD8-) were developed from the peripheral blood of a patient with a combined immunodeficiency and tissue injury resembling graft-vs-host disease. One of these IL-2-dependent T cell lines demonstrated non-MHC-restricted cytolytic function against tumor targets, syngeneic and allogeneic fibroblasts, and PHA blasts from allogeneic donors. The other cell line only became cytotoxic in the presence of lectin or anti-CD3 antibody. The two cell lines also differed in their expression of the T-200 gene products CD45RO (gp180) and CD45RA (gp220). Both cell lines produced tumor necrosis factor-α and -β and IFN-γ activity when activated with mitogens or PMA and IL-1. The in vitro functions of these T-cell lines suggest a potential role for α/β double-negative T lymphocytes in tissue injury resembling graft-vs-host disease.

Original languageEnglish (US)
Pages (from-to)4507-4512
Number of pages6
JournalJournal of Immunology
Volume144
Issue number12
StatePublished - Jun 15 1990
Externally publishedYes

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Lymphokines
T-Cell Antigen Receptor
T-Lymphocytes
Cell Line
Graft vs Host Disease
Wounds and Injuries
Interleukin-1
Mitogens
Lectins
Interleukin-2
Anti-Idiotypic Antibodies
Tumor Necrosis Factor-alpha
Fibroblasts
Tissue Donors
Phenotype
Genes
Neoplasms

ASJC Scopus subject areas

  • Immunology

Cite this

Brooks, E. G., Wirt, D. P., Goldblum, R. M., Vaidya, S., Asuncion, M. T., Patterson, J. C., ... Klimpel, G. R. (1990). Double-negative (CD4- CD8-) T cells with an α/β T cell receptor: Non-MHC-restricted cytolytic activity and lymphokine production. Journal of Immunology, 144(12), 4507-4512.

Double-negative (CD4- CD8-) T cells with an α/β T cell receptor : Non-MHC-restricted cytolytic activity and lymphokine production. / Brooks, Edward G; Wirt, Daniel P.; Goldblum, Randall M.; Vaidya, Smita; Asuncion, Maria T.; Patterson, James C.; Ware, Carl F.; Klimpel, Gary R.

In: Journal of Immunology, Vol. 144, No. 12, 15.06.1990, p. 4507-4512.

Research output: Contribution to journalArticle

Brooks, EG, Wirt, DP, Goldblum, RM, Vaidya, S, Asuncion, MT, Patterson, JC, Ware, CF & Klimpel, GR 1990, 'Double-negative (CD4- CD8-) T cells with an α/β T cell receptor: Non-MHC-restricted cytolytic activity and lymphokine production', Journal of Immunology, vol. 144, no. 12, pp. 4507-4512.
Brooks, Edward G ; Wirt, Daniel P. ; Goldblum, Randall M. ; Vaidya, Smita ; Asuncion, Maria T. ; Patterson, James C. ; Ware, Carl F. ; Klimpel, Gary R. / Double-negative (CD4- CD8-) T cells with an α/β T cell receptor : Non-MHC-restricted cytolytic activity and lymphokine production. In: Journal of Immunology. 1990 ; Vol. 144, No. 12. pp. 4507-4512.
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