Doslidzhennia ekspresiï dezoksyrybonukleaz u klitynakh ssavtsiv metodom renaturatsiï bilkiv u poliakrylamidnomu heli.

I. I. Kit; H. I. Shuvaieva; V. R. Drel'; N. I. Ihumentseva; L. B. Drobot

Doslidzhennia ekspresiï dezoksyrybonukleaz u klitynakh ssavtsiv metodom renaturatsiï bilkiv u poliakrylamidnomu heli.

Translated title of the contribution: Study of expression of desoxyribonucleases in mammalian cells by a protein renaturation method in polyacrylamide gel

I. I. Kit, H. I. Shuvaieva, V. R. Drel', N. I. Ihumentseva, L. B. Drobot

Research output: Contribution to journal › Article › peer-review

Abstract

Analysis of enzymatic activity in polyacrylamide gel is based on highly effective separation of proteins by SDS-electrophoresis with their subsequent renaturation and detection of enzymatic activity. This method was used to study an expression of DNAases in culturing of cells HEK293, NIH 3T3, U937. We have found that in HEK293 cells the nucleases with molecular weights 47 and 45 kDa were expressed. The localization of DNAases in the cell nuclei was shown as well. Induction of apoptosis in HEK293 cells increase the level of p47 DNAase and causes the expression of novel 50 kDa DNAase. We suggested that those discovered DNAases could take part in apoptotic DNA degradation.

Translated title of the contribution	Study of expression of desoxyribonucleases in mammalian cells by a protein renaturation method in polyacrylamide gel
Original language	Ukrainian
Pages (from-to)	116-119
Number of pages	4
Journal	Ukrainski¿ biokhimicheski¿ zhurnal
Volume	74
Issue number	3
State	Published - 2002
Externally published	Yes

ASJC Scopus subject areas

Biochemistry

Cite this

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title = "Doslidzhennia ekspresi{\"i} dezoksyrybonukleaz u klitynakh ssavtsiv metodom renaturatsi{\"i} bilkiv u poliakrylamidnomu heli.",

abstract = "Analysis of enzymatic activity in polyacrylamide gel is based on highly effective separation of proteins by SDS-electrophoresis with their subsequent renaturation and detection of enzymatic activity. This method was used to study an expression of DNAases in culturing of cells HEK293, NIH 3T3, U937. We have found that in HEK293 cells the nucleases with molecular weights 47 and 45 kDa were expressed. The localization of DNAases in the cell nuclei was shown as well. Induction of apoptosis in HEK293 cells increase the level of p47 DNAase and causes the expression of novel 50 kDa DNAase. We suggested that those discovered DNAases could take part in apoptotic DNA degradation.",

author = "Kit, {I. I.} and Shuvaieva, {H. I.} and Drel', {V. R.} and Ihumentseva, {N. I.} and Drobot, {L. B.}",

year = "2002",

language = "Ukrainian",

volume = "74",

pages = "116--119",

journal = "Ukrainski¿ biokhimicheski¿ zhurnal",

issn = "0201-8470",

publisher = "Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine (NASU)",

number = "3",

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TY - JOUR

T1 - Doslidzhennia ekspresiï dezoksyrybonukleaz u klitynakh ssavtsiv metodom renaturatsiï bilkiv u poliakrylamidnomu heli.

AU - Kit, I. I.

AU - Shuvaieva, H. I.

AU - Drel', V. R.

AU - Ihumentseva, N. I.

AU - Drobot, L. B.

PY - 2002

Y1 - 2002

N2 - Analysis of enzymatic activity in polyacrylamide gel is based on highly effective separation of proteins by SDS-electrophoresis with their subsequent renaturation and detection of enzymatic activity. This method was used to study an expression of DNAases in culturing of cells HEK293, NIH 3T3, U937. We have found that in HEK293 cells the nucleases with molecular weights 47 and 45 kDa were expressed. The localization of DNAases in the cell nuclei was shown as well. Induction of apoptosis in HEK293 cells increase the level of p47 DNAase and causes the expression of novel 50 kDa DNAase. We suggested that those discovered DNAases could take part in apoptotic DNA degradation.

AB - Analysis of enzymatic activity in polyacrylamide gel is based on highly effective separation of proteins by SDS-electrophoresis with their subsequent renaturation and detection of enzymatic activity. This method was used to study an expression of DNAases in culturing of cells HEK293, NIH 3T3, U937. We have found that in HEK293 cells the nucleases with molecular weights 47 and 45 kDa were expressed. The localization of DNAases in the cell nuclei was shown as well. Induction of apoptosis in HEK293 cells increase the level of p47 DNAase and causes the expression of novel 50 kDa DNAase. We suggested that those discovered DNAases could take part in apoptotic DNA degradation.

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AN - SCOPUS:0642344230

SN - 0201-8470

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JF - Ukrainski¿ biokhimicheski¿ zhurnal

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ER -

Doslidzhennia ekspresiï dezoksyrybonukleaz u klitynakh ssavtsiv metodom renaturatsiï bilkiv u poliakrylamidnomu heli.

Abstract

ASJC Scopus subject areas

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