Dose response studies on the ability of 7,12 dimethylbenz[a]anthracene and benz[a]anthracene to initiate skin tumors

Thomas J Slaga, G. T. Bowden, J. D. Scribner, R. K. Boutwell

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63 Citations (Scopus)

Abstract

Dose response curves depicting the tumor initiating abilities of the powerful carcinogen 7,12 dimethylbenz[a]anthracene (DMBA) and the supposedly weak carcinogen or noncarcinogen benz[a]anthracene (BA) applied to Charles River CD 1 female mice, revealed that: a single topical dose of either 0.5, 5, 10, 20, 100, or 200 nmoles DMBA, followed 1 wk later by twice weekly applications of 0.25% croton oil, induced 0.4, 2.3, 6.0, 5.5, 6.2, and 16 papillomas per mouse, respectively, at 24 wk. One topical application of 5, 40, or 200 nmoles DMBA, followed by twice weekly applications of 17 nmoles 12O tetradecanoylphorbol 13 acetate (TPA), produced 3.8, 15, and 26 papillomas per mouse at 20 wk after promotion. When DMBA was injected intraperitoneally in amounts of 0.6, 1.2, 2.4, or 4.8 μmoles and followed by twice weekly TPA promotion, the mice developed 7.8, 11.8, 11.6, and 4.4 papillomas per mouse at 20 wk. BA initiated papillomas in 50% of mice by 15 wk after a single dose of 2.2 μmoles BA followed by twice weekly applications of 16 nmoles TPA. A single topical application of 1.1, 2.2, 4.4, or 8.8 μmoles BA, followed by promotion, induced 0.9, 1.6, 2.6, and 2.9 papillomas per mouse by 24 wk. Results suggested that every complete carcinogen has both initiating and promoting abilities, whereas certain weakly carcinogenic or noncarcinogenic polycyclic hydrocarbons may have either initiating or promoting activities.

Original languageEnglish (US)
Pages (from-to)1337-1340
Number of pages4
JournalJournal of the National Cancer Institute
Volume53
Issue number5
StatePublished - 1974
Externally publishedYes

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Papilloma
9,10-Dimethyl-1,2-benzanthracene
Skin
Tetradecanoylphorbol Acetate
Neoplasms
Carcinogens
Cyclic Hydrocarbons
Croton Oil
benz(a)anthracene
Rivers

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Dose response studies on the ability of 7,12 dimethylbenz[a]anthracene and benz[a]anthracene to initiate skin tumors. / Slaga, Thomas J; Bowden, G. T.; Scribner, J. D.; Boutwell, R. K.

In: Journal of the National Cancer Institute, Vol. 53, No. 5, 1974, p. 1337-1340.

Research output: Contribution to journalArticle

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