Dorsal motor vagal neurons can elicit bradycardia and reduce anxiety-like behavior

Misty M. Strain, Nicholas J. Conley, Lily S. Kauffman, Liliana Espinoza, Stephanie Fedorchak, Patricia Castro Martinez, Maisie E. Crook, Maira Jalil, Georgia E. Hodes, Stephen B.G. Abbott, Ali D. Güler, John N. Campbell, Carie R. Boychuk

Research output: Contribution to journalArticlepeer-review

Abstract

Cardiovagal neurons (CVNs) innervate cardiac ganglia through the vagus nerve to control cardiac function. Although the cardioinhibitory role of CVNs in nucleus ambiguus (CVNNA) is well established, the nature and functionality of CVNs in dorsal motor nucleus of the vagus (CVNDMV) is less clear. We therefore aimed to characterize CVNDMV anatomically, physiologically, and functionally. Optogenetically activating cholinergic DMV neurons resulted in robust bradycardia through peripheral muscarinic (parasympathetic) and nicotinic (ganglionic) acetylcholine receptors, but not beta-1-adrenergic (sympathetic) receptors. Retrograde tracing from the cardiac fat pad labeled CVNNA and CVNDMV through the vagus nerve. Using whole-cell patch-clamp, CVNDMV demonstrated greater hyperexcitability and spontaneous action potential firing ex vivo despite similar resting membrane potentials, compared to CVNNA. Chemogenetically activating DMV also caused significant bradycardia with a correlated reduction in anxiety-like behavior. Thus, DMV contains uniquely hyperexcitable CVNs and is capable of cardioinhibition and robust anxiolysis.

Original languageEnglish (US)
Article number109137
JournaliScience
Volume27
Issue number3
DOIs
StatePublished - Mar 15 2024

Keywords

  • Behavioral neuroscience
  • Biological sciences
  • Natural sciences
  • Neuroscience
  • Physiology
  • Systems neuroscience

ASJC Scopus subject areas

  • General

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