Dopaminergic stimulation of cAMP accumulation in cultured rat mesangial cells

P. J. Shultz, J. R. Sedor, H. E. Abboud

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Dopamine (DA) alters renal hemodynamics, and DA receptors have been demonstrated in isolated glomeruli. To determine the glomerular cell type bearing DA receptors, we studied the effect of dopaminergic agonists and antagonists on adenosine 3',5'-cyclic monophosphate (cAMP) accumulation in rat glomerular mesangial and epithelial cells in culture. DA caused a marked dose- and time-dependent increase in cAMP accumulation in mesangial but not epithelial cells. The stimulatory effect of DA was abolished by the DA antagonists haloperidol, trifluoperazine, and cis-thiothixene but not by β- or α-adrenergic or histamine antagonists. Similar to the effects of DA, two dopamine type I receptor agonists, fenoldopam and SKF 38393, markedly stimulated cAMP accumulation in the mesangial cells. Moreover, the effects of DA were blocked by Sch 23390, a specific DA1 receptor antagonist, but not domperidone, a specific DA2 antagonist. These results show that DA regulates cAMP accumulation in mesangial cells via DA1-type receptors.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume253
Issue number2
StatePublished - 1987
Externally publishedYes

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Mesangial Cells
Dopamine
Dopamine Antagonists
Dopamine Agonists
Dopamine Receptors
Thiothixene
Fenoldopam
Epithelial Cells
2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
Domperidone
Trifluoperazine
Adrenergic Antagonists
Histamine Antagonists
Haloperidol
Cyclic AMP
Cell Culture Techniques
Hemodynamics
Kidney

ASJC Scopus subject areas

  • Physiology

Cite this

Dopaminergic stimulation of cAMP accumulation in cultured rat mesangial cells. / Shultz, P. J.; Sedor, J. R.; Abboud, H. E.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 253, No. 2, 1987.

Research output: Contribution to journalArticle

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