Dopamine D2-receptor messenger RNA is differentially regulated by dopaminergic agents in rat anterior and neurointermediate pituitary

Dominic J. Autelitano, Lenore Snyder, Stuart C. Sealfon, James L. Roberts

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

Hypothalamic dopamine (DA), acting at DA D2-receptors (D2-R) on pituitary target cells, mediates peptide release and biosynthesis of rat pituitary anterior lobe (AL) prolactin, and neurointermediate lobe (NIL) pro-opiomelanocortin (POMC). We were interested in determining if dopamine agonists and antagonists were capable of modifying D2-R gene expression in these pituitary cells. Utilizing the recently published sequence of the rat D2-R, we isolated a rat D2-R cDNA clone by polymerase chain reaction, and have synthesized RNA probes to quantitate levels of D2-R mRNA by solution hybridization/nuclease protection assay. We report here that 5-day administration of the DA antagonist haloperidol led to significant increase in both D2-R mRNA and POMC mRNA in the NIL; the DA agonist bromocriptine caused a significant decrease in NIL POMC mRNA with no parallel change in D2-R mRNA. In contrast, no significant changes in D2-R mRNA in AL were observed following treatment with either the DA agonist or antagonist. These data provide evidence for tissue-specific regulation of D2-R mRNA in response to dopaminergic manipulation.

Original languageEnglish (US)
Pages (from-to)101-105
Number of pages5
JournalMolecular and Cellular Endocrinology
Volume67
Issue number1
DOIs
StatePublished - Nov 1989
Externally publishedYes

Keywords

  • Dopamine D-receptor
  • Messenger RNA
  • Pituitary
  • Regulation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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