Donor variation in in vitro HIV-1 susceptibility of monocyte-derived macrophages

Sebastiaan M. Bol, Yvonne van Remmerden, Jantine G. Sietzema, Neeltje A. Kootstra, Hanneke Schuitemaker, Angélique B. van 't Wout

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Primary human cells from different donors vary in their susceptibility to in vitro infection with HIV-1. In order to perform genetic analysis to identify host factors that affect HIV-1 susceptibility, it is important that a clear phenotype is defined. Here, we report a standardized method to study variation for in vitro HIV-1 infection in monocyte-derived macrophages (MDM) from large numbers of individuals. With this assay, HIV-1 susceptibility of MDM from 489 different donors shows more than 3 log variation and a good correlation with the 32 base pair deletion in the CCR5 co-receptor (ccr5 Δ32 genotype) of the donors. However, in 7 of 12 donors completely resistant to infection with CCR5-using HIV-1, this was not explained by the ccr5 Δ32 genotype, showing evidence that other host factors are likely to influence HIV-1 replication in MDM. Infections with VSV-G pseudotyped HIV-1 indeed confirmed the existence of post-entry level restrictions in MDM.

Original languageEnglish (US)
Pages (from-to)205-211
Number of pages7
JournalVirology
Volume390
Issue number2
DOIs
StatePublished - Aug 1 2009
Externally publishedYes

Keywords

  • CCR5
  • HIV-1
  • Host genetic variation
  • Macrophage
  • Monocyte

ASJC Scopus subject areas

  • Virology

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