Domain architecture and oligomerization properties of the paramyxovirus PIV 5 hemagglutinin-neuraminidase (HN) protein

Ping Yuan, George P. Leser, Borries Demeler, Robert A. Lamb, Theodore S. Jardetzky

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

The mechanism by which the paramyxovirus hemagglutinin-neuraminidase (HN) protein couples receptor binding to activation of virus entry remains to be fully understood, but the HN stalk is thought to play an important role in the process. We have characterized ectodomain constructs of the parainfluenza virus 5 HN to understand better the underlying architecture and oligomerization properties that may influence HN functions. The PIV 5 neuraminidase (NA) domain is monomeric whereas the ectodomain forms a well-defined tetramer. The HN stalk also forms tetramers and higher order oligomers with high α-helical content. Together, the data indicate that the globular NA domains form weak intersubunit interactions at the end of the HN stalk tetramer, while stabilizing the stalk and overall oligomeric state of the ectodomain. Electron microscopy of the HN ectodomain reveals flexible arrangements of the NA and stalk domains, which may be important for understanding how these two HN domains impact virus entry.

Original languageEnglish (US)
Pages (from-to)282-291
Number of pages10
JournalVirology
Volume378
Issue number2
DOIs
StatePublished - Sep 1 2008

Keywords

  • Hemagglutinin-neuraminidase (HN)
  • Membrane fusion/HN stalk
  • Parainfluenza virus 5
  • Paramyxovirus
  • Sialic acid
  • Virus entry

ASJC Scopus subject areas

  • Virology

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