Docosahexaenoic Acid Supplementation in Pregnancy Modulates Placental Cellular Signaling and Nutrient Transport Capacity in Obese Women

Susanne Lager, Vanessa I. Ramirez, Ometeotl Acosta, Christiane Meireles, Evelyn Miller, Francesca Gaccioli, Fredrick J. Rosario, Jonathan A Gelfond, Kevin Hakala, Susan E Weintraub, Debra A. Krummel, Theresa L. Powell

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Context: Maternal obesity in pregnancy has profound impacts on maternal metabolism and promotes placental nutrient transport, which may contribute to fetal overgrowth in these pregnancies. The fatty acid docosahexaenoic acid (DHA) has bioactive properties that may improve outcomes in obese pregnant women by modulating placental function.

Objective: To determine the effects of DHA supplementation in obese pregnant women on maternal metabolism and placental function.

Design: Pregnant women were supplemented with DHA or placebo. Maternal fasting blood was collected at 26 and 36 weeks' gestation, and placentas were collected at term.

Setting: Academic health care institution.

Subjects: Thirty-eight pregnant women with pregravid body mass index ≥30 kg/m2.

Intervention: DHA (800 mg, algal oil) or placebo (corn/soy oil) daily from 26 weeks to term.

Main Outcomes: DHA content of maternal erythrocyte and placental membranes, maternal fasting blood glucose, cytokines, metabolic hormones, and circulating lipids were determined. Insulin, mTOR, and inflammatory signaling were assessed in placental homogenates, and nutrient transport capacity was determined in isolated syncytiotrophoblast plasma membranes.

Results: DHA supplementation increased erythrocyte (P < 0.0001) and placental membrane DHA levels (P < 0.0001) but did not influence maternal inflammatory status, insulin sensitivity, or lipids. DHA supplementation decreased placental inflammation, amino acid transporter expression, and activity (P < 0.01) and increased placental protein expression of fatty acid transporting protein 4 (P < 0.05).

Conclusions: Maternal DHA supplementation in pregnancy decreases placental inflammation and differentially modulates placental nutrient transport capacity and may mitigate adverse effects of maternal obesity on placental function.

Original languageEnglish (US)
Pages (from-to)4557-4567
Number of pages11
JournalThe Journal of clinical endocrinology and metabolism
Volume102
Issue number12
DOIs
StatePublished - Dec 1 2017

Fingerprint

Cell signaling
Docosahexaenoic Acids
Nutrients
Mothers
Food
Pregnancy
Pregnant Women
Metabolism
Fasting
Oils
Fatty Acids
Obesity
Placebos
Insulin
Pregnancy Proteins
Inflammation
Membranes
Amino Acid Transport Systems
Lipids
Corn Oil

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Docosahexaenoic Acid Supplementation in Pregnancy Modulates Placental Cellular Signaling and Nutrient Transport Capacity in Obese Women. / Lager, Susanne; Ramirez, Vanessa I.; Acosta, Ometeotl; Meireles, Christiane; Miller, Evelyn; Gaccioli, Francesca; Rosario, Fredrick J.; Gelfond, Jonathan A; Hakala, Kevin; Weintraub, Susan E; Krummel, Debra A.; Powell, Theresa L.

In: The Journal of clinical endocrinology and metabolism, Vol. 102, No. 12, 01.12.2017, p. 4557-4567.

Research output: Contribution to journalArticle

Lager, Susanne ; Ramirez, Vanessa I. ; Acosta, Ometeotl ; Meireles, Christiane ; Miller, Evelyn ; Gaccioli, Francesca ; Rosario, Fredrick J. ; Gelfond, Jonathan A ; Hakala, Kevin ; Weintraub, Susan E ; Krummel, Debra A. ; Powell, Theresa L. / Docosahexaenoic Acid Supplementation in Pregnancy Modulates Placental Cellular Signaling and Nutrient Transport Capacity in Obese Women. In: The Journal of clinical endocrinology and metabolism. 2017 ; Vol. 102, No. 12. pp. 4557-4567.
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abstract = "Context: Maternal obesity in pregnancy has profound impacts on maternal metabolism and promotes placental nutrient transport, which may contribute to fetal overgrowth in these pregnancies. The fatty acid docosahexaenoic acid (DHA) has bioactive properties that may improve outcomes in obese pregnant women by modulating placental function.Objective: To determine the effects of DHA supplementation in obese pregnant women on maternal metabolism and placental function.Design: Pregnant women were supplemented with DHA or placebo. Maternal fasting blood was collected at 26 and 36 weeks' gestation, and placentas were collected at term.Setting: Academic health care institution.Subjects: Thirty-eight pregnant women with pregravid body mass index ≥30 kg/m2.Intervention: DHA (800 mg, algal oil) or placebo (corn/soy oil) daily from 26 weeks to term.Main Outcomes: DHA content of maternal erythrocyte and placental membranes, maternal fasting blood glucose, cytokines, metabolic hormones, and circulating lipids were determined. Insulin, mTOR, and inflammatory signaling were assessed in placental homogenates, and nutrient transport capacity was determined in isolated syncytiotrophoblast plasma membranes.Results: DHA supplementation increased erythrocyte (P < 0.0001) and placental membrane DHA levels (P < 0.0001) but did not influence maternal inflammatory status, insulin sensitivity, or lipids. DHA supplementation decreased placental inflammation, amino acid transporter expression, and activity (P < 0.01) and increased placental protein expression of fatty acid transporting protein 4 (P < 0.05).Conclusions: Maternal DHA supplementation in pregnancy decreases placental inflammation and differentially modulates placental nutrient transport capacity and may mitigate adverse effects of maternal obesity on placental function.",
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T1 - Docosahexaenoic Acid Supplementation in Pregnancy Modulates Placental Cellular Signaling and Nutrient Transport Capacity in Obese Women

AU - Lager, Susanne

AU - Ramirez, Vanessa I.

AU - Acosta, Ometeotl

AU - Meireles, Christiane

AU - Miller, Evelyn

AU - Gaccioli, Francesca

AU - Rosario, Fredrick J.

AU - Gelfond, Jonathan A

AU - Hakala, Kevin

AU - Weintraub, Susan E

AU - Krummel, Debra A.

AU - Powell, Theresa L.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Context: Maternal obesity in pregnancy has profound impacts on maternal metabolism and promotes placental nutrient transport, which may contribute to fetal overgrowth in these pregnancies. The fatty acid docosahexaenoic acid (DHA) has bioactive properties that may improve outcomes in obese pregnant women by modulating placental function.Objective: To determine the effects of DHA supplementation in obese pregnant women on maternal metabolism and placental function.Design: Pregnant women were supplemented with DHA or placebo. Maternal fasting blood was collected at 26 and 36 weeks' gestation, and placentas were collected at term.Setting: Academic health care institution.Subjects: Thirty-eight pregnant women with pregravid body mass index ≥30 kg/m2.Intervention: DHA (800 mg, algal oil) or placebo (corn/soy oil) daily from 26 weeks to term.Main Outcomes: DHA content of maternal erythrocyte and placental membranes, maternal fasting blood glucose, cytokines, metabolic hormones, and circulating lipids were determined. Insulin, mTOR, and inflammatory signaling were assessed in placental homogenates, and nutrient transport capacity was determined in isolated syncytiotrophoblast plasma membranes.Results: DHA supplementation increased erythrocyte (P < 0.0001) and placental membrane DHA levels (P < 0.0001) but did not influence maternal inflammatory status, insulin sensitivity, or lipids. DHA supplementation decreased placental inflammation, amino acid transporter expression, and activity (P < 0.01) and increased placental protein expression of fatty acid transporting protein 4 (P < 0.05).Conclusions: Maternal DHA supplementation in pregnancy decreases placental inflammation and differentially modulates placental nutrient transport capacity and may mitigate adverse effects of maternal obesity on placental function.

AB - Context: Maternal obesity in pregnancy has profound impacts on maternal metabolism and promotes placental nutrient transport, which may contribute to fetal overgrowth in these pregnancies. The fatty acid docosahexaenoic acid (DHA) has bioactive properties that may improve outcomes in obese pregnant women by modulating placental function.Objective: To determine the effects of DHA supplementation in obese pregnant women on maternal metabolism and placental function.Design: Pregnant women were supplemented with DHA or placebo. Maternal fasting blood was collected at 26 and 36 weeks' gestation, and placentas were collected at term.Setting: Academic health care institution.Subjects: Thirty-eight pregnant women with pregravid body mass index ≥30 kg/m2.Intervention: DHA (800 mg, algal oil) or placebo (corn/soy oil) daily from 26 weeks to term.Main Outcomes: DHA content of maternal erythrocyte and placental membranes, maternal fasting blood glucose, cytokines, metabolic hormones, and circulating lipids were determined. Insulin, mTOR, and inflammatory signaling were assessed in placental homogenates, and nutrient transport capacity was determined in isolated syncytiotrophoblast plasma membranes.Results: DHA supplementation increased erythrocyte (P < 0.0001) and placental membrane DHA levels (P < 0.0001) but did not influence maternal inflammatory status, insulin sensitivity, or lipids. DHA supplementation decreased placental inflammation, amino acid transporter expression, and activity (P < 0.01) and increased placental protein expression of fatty acid transporting protein 4 (P < 0.05).Conclusions: Maternal DHA supplementation in pregnancy decreases placental inflammation and differentially modulates placental nutrient transport capacity and may mitigate adverse effects of maternal obesity on placental function.

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DO - 10.1210/jc.2017-01384

M3 - Article

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SP - 4557

EP - 4567

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

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