TY - JOUR
T1 - Docosahexaenoic Acid (DHA) Supplementation Alters Phospholipid Species and Lipid Peroxidation Products in Adult Mouse Brain, Heart, and Plasma
AU - Sun, Grace Y.
AU - Appenteng, Michael K.
AU - Li, Runting
AU - Woo, Taeseon
AU - Yang, Bo
AU - Qin, Chao
AU - Pan, Meixia
AU - Cieślik, Magdalena
AU - Cui, Jiankun
AU - Fritsche, Kevin L.
AU - Gu, Zezong
AU - Will, Matthew
AU - Beversdorf, David
AU - Adamczyk, Agata
AU - Han, Xianlin
AU - Greenlief, C. Michael
N1 - Funding Information:
This study is supported by the MU Research Council grant (17–009) and support from Department of Radiology Mission Enhancement funds from University of Missouri to DQB, and NIH/NIA RF1 AG061872 to XH. We wish to thank DSM Nutritional Products (USA) for donating DHASCO, the source of dietary DHA used in this study. MC’s visit to University of Missouri was funded in part by a scholarship for young scientists from the Polish Academy of Sciences and Mossakowski Medical Research Centre, Poland.
Publisher Copyright:
© 2020, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2021/3
Y1 - 2021/3
N2 - The abundance of docosahexaenoic acid (DHA) in phospholipids in the brain and retina has generated interest to search for its role in mediating neurological functions. Besides the source of many oxylipins with pro-resolving properties, DHA also undergoes peroxidation, producing 4-hydroxyhexenal (4-HHE), although its function remains elusive. Despite wide dietary consumption, whether supplementation of DHA may alter the peroxidation products and their relationship to phospholipid species in brain and other body organs have not been explored sufficiently. In this study, adult mice were administered a control or DHA-enriched diet for 3 weeks, and phospholipid species and peroxidation products were examined in brain, heart, and plasma. Results demonstrated that this dietary regimen increased (n-3) and decreased (n-6) species to different extent in all major phospholipid classes (PC, dPE, PE-pl, PI and PS) examined. Besides changes in phospholipid species, DHA-enriched diet also showed substantial increases in 4-HHE in brain, heart, and plasma. Among different brain regions, the hippocampus responded to the DHA-enriched diet showing significant increase in 4-HHE. Considering the pro- and anti-inflammatory pathways mediated by the (n-6) and (n-3) polyunsaturated fatty acids, unveiling the ability for DHA-enriched diet to alter phospholipid species and lipid peroxidation products in the brain and in different body organs may be an important step forward towards understanding the mechanism(s) for this (n-3) fatty acid on health and diseases.
AB - The abundance of docosahexaenoic acid (DHA) in phospholipids in the brain and retina has generated interest to search for its role in mediating neurological functions. Besides the source of many oxylipins with pro-resolving properties, DHA also undergoes peroxidation, producing 4-hydroxyhexenal (4-HHE), although its function remains elusive. Despite wide dietary consumption, whether supplementation of DHA may alter the peroxidation products and their relationship to phospholipid species in brain and other body organs have not been explored sufficiently. In this study, adult mice were administered a control or DHA-enriched diet for 3 weeks, and phospholipid species and peroxidation products were examined in brain, heart, and plasma. Results demonstrated that this dietary regimen increased (n-3) and decreased (n-6) species to different extent in all major phospholipid classes (PC, dPE, PE-pl, PI and PS) examined. Besides changes in phospholipid species, DHA-enriched diet also showed substantial increases in 4-HHE in brain, heart, and plasma. Among different brain regions, the hippocampus responded to the DHA-enriched diet showing significant increase in 4-HHE. Considering the pro- and anti-inflammatory pathways mediated by the (n-6) and (n-3) polyunsaturated fatty acids, unveiling the ability for DHA-enriched diet to alter phospholipid species and lipid peroxidation products in the brain and in different body organs may be an important step forward towards understanding the mechanism(s) for this (n-3) fatty acid on health and diseases.
KW - Docosahexaenoic acid
KW - Heart
KW - Hippocampus
KW - Lipid peroxidation
KW - Lipidomics
KW - Plasma
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U2 - 10.1007/s12017-020-08616-0
DO - 10.1007/s12017-020-08616-0
M3 - Article
C2 - 32926329
AN - SCOPUS:85090972584
SN - 1535-1084
VL - 23
SP - 118
EP - 129
JO - NeuroMolecular Medicine
JF - NeuroMolecular Medicine
IS - 1
ER -