DNA subtypes and fluconazole susceptibilities of candida albicans isolates from the oral cavities of patients with AIDS

Francesco Barchiesi, Richard J. Hollis, Deanna A. McGough, Giorgio Scalise, Michael G. Rinaldi, Michael A. Pfaller

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

Sixty-two Candida albicans isolates from the oral cavities of 28 patients with AIDS who were receiving fluconazole therapy were typed by restriction endonuclease analysis followed by pulsed-field gel electrophoresis; these isolates were then tested for fluconazole susceptibility by a standard broth dilution method. Sequential isolates (range, 2–4) were evaluated for 22 patients; only one isolate was evaluated for six patients. DNA subtyping revealed a total of 37 different DNA subtypes. Twelve (54.5%) of 22 patients with multiple episodes of oropharyngeal candidiasis were infected with a single DNA subtype throughout the observation period. Ten (45.5%) of 22 patients with multiple episodes of oropharyngeal candidiasis were infected with two or three DNA subtypes during the observation period. In vitro susceptibility tests revealed that MICs of fluconazole ranged from ≤0.125 μg/mL to 64 μg/mL, with an MIC50 of 0.5 μg/mL and an MIC90 of 4 μg/mL. A significant increase in the MICs (fourfold or greater) of fluconazole for sequential C. albicans isolates was found for 66.6% of the patients infected with a single DNA inftype and for 50% of the patients infected with multiple DNA inftypes. Despite a limited number of patients and isolates, our data suggest that C. albicans isolates that are susceptible to fluconazole at MICs of ≥8 μg/mL in vitro will be less susceptible in vivo to standard doses (100–200 mg/d) of this drug.

Original languageEnglish (US)
Pages (from-to)634-640
Number of pages7
JournalClinical Infectious Diseases
Volume20
Issue number3
DOIs
StatePublished - Mar 1995
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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