DNA probes for identifying chromosomes 5, 6, and 7 of Schistosoma mansoni

T. Taguchi; Y. Hirai; P. T. LoVerde; A. Tominaga; H. Hirai

doi:10.1645/GE-1099R.1

DNA probes for identifying chromosomes 5, 6, and 7 of Schistosoma mansoni

T. Taguchi, Y. Hirai, P. T. LoVerde, A. Tominaga, H. Hirai

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Schistosoma mansoni has a genome of 270 Mb contained on 8 pairs of chromosomes. C-banding has been a useful technique in identifying the 7 autosomal and sex chromosomes. However, even with C-banding, S. mansoni chromosomes 5, 6, and 7 are difficult to discriminate from each other, because of their small sizes, morphological similarity and poor banding patterns. We have identified probes that specifically paint chromosomes 5, 6, and 7 of S. mansoni with the use of chromosome microdissection and the degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). Exact chromosome identification is required for accurate chromosome mapping of genomic clones and genetic elements, which is an essential component of the schistosome genome project.

Original language	English (US)
Pages (from-to)	724-726
Number of pages	3
Journal	Journal of Parasitology
Volume	93
Issue number	3
DOIs	https://doi.org/10.1645/GE-1099R.1
State	Published - Jun 2007
Externally published	Yes

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Parasitology

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abstract = "Schistosoma mansoni has a genome of 270 Mb contained on 8 pairs of chromosomes. C-banding has been a useful technique in identifying the 7 autosomal and sex chromosomes. However, even with C-banding, S. mansoni chromosomes 5, 6, and 7 are difficult to discriminate from each other, because of their small sizes, morphological similarity and poor banding patterns. We have identified probes that specifically paint chromosomes 5, 6, and 7 of S. mansoni with the use of chromosome microdissection and the degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). Exact chromosome identification is required for accurate chromosome mapping of genomic clones and genetic elements, which is an essential component of the schistosome genome project.",

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T1 - DNA probes for identifying chromosomes 5, 6, and 7 of Schistosoma mansoni

AU - Taguchi, T.

AU - Hirai, Y.

AU - LoVerde, P. T.

AU - Tominaga, A.

AU - Hirai, H.

PY - 2007/6

Y1 - 2007/6

N2 - Schistosoma mansoni has a genome of 270 Mb contained on 8 pairs of chromosomes. C-banding has been a useful technique in identifying the 7 autosomal and sex chromosomes. However, even with C-banding, S. mansoni chromosomes 5, 6, and 7 are difficult to discriminate from each other, because of their small sizes, morphological similarity and poor banding patterns. We have identified probes that specifically paint chromosomes 5, 6, and 7 of S. mansoni with the use of chromosome microdissection and the degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). Exact chromosome identification is required for accurate chromosome mapping of genomic clones and genetic elements, which is an essential component of the schistosome genome project.

AB - Schistosoma mansoni has a genome of 270 Mb contained on 8 pairs of chromosomes. C-banding has been a useful technique in identifying the 7 autosomal and sex chromosomes. However, even with C-banding, S. mansoni chromosomes 5, 6, and 7 are difficult to discriminate from each other, because of their small sizes, morphological similarity and poor banding patterns. We have identified probes that specifically paint chromosomes 5, 6, and 7 of S. mansoni with the use of chromosome microdissection and the degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). Exact chromosome identification is required for accurate chromosome mapping of genomic clones and genetic elements, which is an essential component of the schistosome genome project.

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DNA probes for identifying chromosomes 5, 6, and 7 of Schistosoma mansoni

Abstract

ASJC Scopus subject areas

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