DNA probes for identifying chromosomes 5, 6, and 7 of Schistosoma mansoni

T. Taguchi, Y. Hirai, P. T. LoVerde, A. Tominaga, H. Hirai

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Schistosoma mansoni has a genome of 270 Mb contained on 8 pairs of chromosomes. C-banding has been a useful technique in identifying the 7 autosomal and sex chromosomes. However, even with C-banding, S. mansoni chromosomes 5, 6, and 7 are difficult to discriminate from each other, because of their small sizes, morphological similarity and poor banding patterns. We have identified probes that specifically paint chromosomes 5, 6, and 7 of S. mansoni with the use of chromosome microdissection and the degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). Exact chromosome identification is required for accurate chromosome mapping of genomic clones and genetic elements, which is an essential component of the schistosome genome project.

Original languageEnglish (US)
Pages (from-to)724-726
Number of pages3
JournalJournal of Parasitology
Volume93
Issue number3
DOIs
StatePublished - Jun 1 2007

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ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Taguchi, T., Hirai, Y., LoVerde, P. T., Tominaga, A., & Hirai, H. (2007). DNA probes for identifying chromosomes 5, 6, and 7 of Schistosoma mansoni. Journal of Parasitology, 93(3), 724-726. https://doi.org/10.1645/GE-1099R.1