DNA packaging in vitro by an isolated bacteriophage T7 procapsid

W. E. Masker, P. Serwer

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The results of previous in vivo studies indicate that a DNA-free procapsid (capsid I) packages bacteriophage T7 DNA during infection of Escherichia coli. It was shown here that capsid I, isolated by electrophoresis in metrizamide density gradients, packaged DNA and formed infectious phage particles when incubated in vitro with extracts deficient in capsid proteins.

Original languageEnglish (US)
Pages (from-to)1138-1142
Number of pages5
JournalJournal of virology
Volume43
Issue number3
DOIs
StatePublished - 1982
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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