TY - JOUR
T1 - DNA double-strand breaks and micronuclei in human blood lymphocytes after repeated whole body exposures to 7T Magnetic Resonance Imaging
AU - Fatahi, Mahsa
AU - Reddig, Annika
AU - Vijayalaxmi,
AU - Friebe, Björn
AU - Hartig, Roland
AU - Prihoda, Thomas J.
AU - Ricke, Jens
AU - Roggenbuck, Dirk
AU - Reinhold, Dirk
AU - Speck, Oliver
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Purpose: To examine the extent of genetic damage, assessed from deoxyribonucleic acid (DNA) double-strand breaks (DSBs) and micronuclei (MN) in peripheral blood mononuclear cells obtained from individuals repeatedly exposed to 7T Magnetic Resonance Imaging (MRI). Materials and methods: The study protocol was approved by the local ethics committee. Informed consent was obtained from 22 healthy, non-smoking, non-alcoholic male individuals, who had never undergone radio-/chemo-therapy, scintigraphy, and had not undergone X-ray examination one year prior blood withdrawal. Eleven participants were repeatedly exposed to 7T and 3T MRI while working with/around scanners or frequently participating as 7T and lower field MRI research subjects (mean age 34 ± 7 years). The other half was never exposed to 7T or lower field MRI and served as controls (mean age 33 ± 9 years).The damage in lymphocytes was assessed using anti-γH2AX immunofluorescence staining of DNA DSBs and by quantification of MN. Isolated cells were further exposed in vitro to 7T MRI either alone or in the presence of the DNA damaging drug etoposide, to determine if there is any additional combined effect. The kinetics of DNA damage repair were examined. Results: The mean base-level of γH2AX foci/cell and incidence of MN between repeatedly exposed and control group were not significantly different (P = 0.618 and P = 0.535, respectively). The additional in vitro exposure of cells to 7T MRI had no significant impact on MN frequencies and γH2AX foci at 1, 20 and 72 h after exposure. Conclusion: Frequently repeated 7T MRI exposure did not result in a detectable increase in genotoxicity indices and alterations of DNA repair kinetics.
AB - Purpose: To examine the extent of genetic damage, assessed from deoxyribonucleic acid (DNA) double-strand breaks (DSBs) and micronuclei (MN) in peripheral blood mononuclear cells obtained from individuals repeatedly exposed to 7T Magnetic Resonance Imaging (MRI). Materials and methods: The study protocol was approved by the local ethics committee. Informed consent was obtained from 22 healthy, non-smoking, non-alcoholic male individuals, who had never undergone radio-/chemo-therapy, scintigraphy, and had not undergone X-ray examination one year prior blood withdrawal. Eleven participants were repeatedly exposed to 7T and 3T MRI while working with/around scanners or frequently participating as 7T and lower field MRI research subjects (mean age 34 ± 7 years). The other half was never exposed to 7T or lower field MRI and served as controls (mean age 33 ± 9 years).The damage in lymphocytes was assessed using anti-γH2AX immunofluorescence staining of DNA DSBs and by quantification of MN. Isolated cells were further exposed in vitro to 7T MRI either alone or in the presence of the DNA damaging drug etoposide, to determine if there is any additional combined effect. The kinetics of DNA damage repair were examined. Results: The mean base-level of γH2AX foci/cell and incidence of MN between repeatedly exposed and control group were not significantly different (P = 0.618 and P = 0.535, respectively). The additional in vitro exposure of cells to 7T MRI had no significant impact on MN frequencies and γH2AX foci at 1, 20 and 72 h after exposure. Conclusion: Frequently repeated 7T MRI exposure did not result in a detectable increase in genotoxicity indices and alterations of DNA repair kinetics.
KW - Double strand breaks
KW - Human blood lymphocytes
KW - Micronuclei
KW - Repeated exposure
KW - Ultra-High Field Magnetic Resonance Imaging
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U2 - 10.1016/j.neuroimage.2016.03.023
DO - 10.1016/j.neuroimage.2016.03.023
M3 - Article
C2 - 26994830
AN - SCOPUS:84962018455
VL - 133
SP - 288
EP - 293
JO - NeuroImage
JF - NeuroImage
SN - 1053-8119
ER -