DNA double-strand breaks and micronuclei in human blood lymphocytes after repeated whole body exposures to 7T Magnetic Resonance Imaging

Mahsa Fatahi, Annika Reddig, Vijayalaxmi, Björn Friebe, Roland Hartig, Thomas J. Prihoda, Jens Ricke, Dirk Roggenbuck, Dirk Reinhold, Oliver Speck

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Purpose: To examine the extent of genetic damage, assessed from deoxyribonucleic acid (DNA) double-strand breaks (DSBs) and micronuclei (MN) in peripheral blood mononuclear cells obtained from individuals repeatedly exposed to 7T Magnetic Resonance Imaging (MRI). Materials and methods: The study protocol was approved by the local ethics committee. Informed consent was obtained from 22 healthy, non-smoking, non-alcoholic male individuals, who had never undergone radio-/chemo-therapy, scintigraphy, and had not undergone X-ray examination one year prior blood withdrawal. Eleven participants were repeatedly exposed to 7T and 3T MRI while working with/around scanners or frequently participating as 7T and lower field MRI research subjects (mean age 34 ± 7 years). The other half was never exposed to 7T or lower field MRI and served as controls (mean age 33 ± 9 years).The damage in lymphocytes was assessed using anti-γH2AX immunofluorescence staining of DNA DSBs and by quantification of MN. Isolated cells were further exposed in vitro to 7T MRI either alone or in the presence of the DNA damaging drug etoposide, to determine if there is any additional combined effect. The kinetics of DNA damage repair were examined. Results: The mean base-level of γH2AX foci/cell and incidence of MN between repeatedly exposed and control group were not significantly different (P = 0.618 and P = 0.535, respectively). The additional in vitro exposure of cells to 7T MRI had no significant impact on MN frequencies and γH2AX foci at 1, 20 and 72 h after exposure. Conclusion: Frequently repeated 7T MRI exposure did not result in a detectable increase in genotoxicity indices and alterations of DNA repair kinetics.

Original languageEnglish (US)
Pages (from-to)288-293
Number of pages6
JournalNeuroImage
Volume133
DOIs
StatePublished - Jun 1 2016

Keywords

  • Double strand breaks
  • Human blood lymphocytes
  • Micronuclei
  • Repeated exposure
  • Ultra-High Field Magnetic Resonance Imaging

ASJC Scopus subject areas

  • Neurology
  • Cognitive Neuroscience

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    Fatahi, M., Reddig, A., Vijayalaxmi, Friebe, B., Hartig, R., Prihoda, T. J., Ricke, J., Roggenbuck, D., Reinhold, D., & Speck, O. (2016). DNA double-strand breaks and micronuclei in human blood lymphocytes after repeated whole body exposures to 7T Magnetic Resonance Imaging. NeuroImage, 133, 288-293. https://doi.org/10.1016/j.neuroimage.2016.03.023