DNA binding site of the growth factor-inducible protein Zif268

B. Christy, D. Nathans

Research output: Contribution to journalArticlepeer-review

441 Scopus citations

Abstract

Zif268, a zinc finger protein whose mRNA is rapidly activated in cells exposed to growth factors or other signaling agents, is thought to play a role in regulating the genetic program induced by extracellular ligands. We report that Zif268 has one of the characteristics of a transcriptional regulator, namely, sequence-specific binding to DNA. Zif268 synthesized in Escherichia coli bound to two sites upstream of the zif268 gene and to sites in the promoter regions of other genes. The nucleotide sequences responsible for binding were defined by DNase I footprinting, by methylation interference experiments, and by use of synthetic oligonucleotides.

Original languageEnglish (US)
Pages (from-to)8737-8741
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number22
DOIs
StatePublished - 1989

Keywords

  • cell growth
  • transcription factor
  • zinc fingers

ASJC Scopus subject areas

  • General

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