Distribution of the folate receptor GP38 in normal and malignant cell lines and tissues

Steven D Weitman, Richard H. Lark, Leslie R. Coney, Daniel W. Fort, Verna Frasca, Vincent R. Zurawski, Barton A. Kamen

Research output: Contribution to journalArticle

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Abstract

In some epithelial cells studied in vitro a membrane-bound folate receptor initiates the process for cell accumulation of 5-methyltetrahydrofolic acid. This receptor was found to be GP38, an overexpressed, glycosyl-phosphatidylinositol anchored glycoprotein, recognized by two monoclonal antibodies, designated MOv18 and MOv19. Using immunoblotting with MOv19, radioimmunoassay with MOv18 and 19, Northern blot analysis, and radioligand binding when possible, we describe the limited expression of the folate receptor in a large number of normal tissues from four autopsies. The immunoblot technique detected as little as 40 pg (≈1 fmol) of receptor protein. Choroid plexus consistently had the largest amount of folate receptor. Other tissues containing substantial amounts of receptor included lung, thyroid, and kidney. The liver, intestines, muscle, cerebellum, cerebrum, and spinal cord were immunologically nonreactive. Folate receptor gene expression determined by Northern blot analysis confirmed these observations. We also show that several malignant cell lines express significantly more receptor than normal epithelial cells or fibroblasts. Specifically, malignant cells bound ≥20 pmol [3H]folate/106 cells, while normal epithelial cells and fibroblasts bound ≤1 pmol radioligand/106 cells. We also demonstrate that 4 of 6 brain tumors overexpress the folate receptor. These studies reveal the limited normal tissue distribution of the folate receptor, a cell surface protein which may be a useful immunological or pharmacological target for the development of selective cancer therapy.

Original languageEnglish (US)
Pages (from-to)3396-3401
Number of pages6
JournalCancer Research
Volume52
Issue number12
StatePublished - Jun 15 1992
Externally publishedYes

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Folic Acid
Cell Line
Epithelial Cells
Northern Blotting
Fibroblasts
Glycosylphosphatidylinositols
Choroid Plexus
Normal Distribution
Cerebrum
Tissue Distribution
Immunoblotting
Brain Neoplasms
Cerebellum
Intestines
Radioimmunoassay
Autopsy
Spinal Cord
Glycoproteins
Thyroid Gland
Membrane Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Weitman, S. D., Lark, R. H., Coney, L. R., Fort, D. W., Frasca, V., Zurawski, V. R., & Kamen, B. A. (1992). Distribution of the folate receptor GP38 in normal and malignant cell lines and tissues. Cancer Research, 52(12), 3396-3401.

Distribution of the folate receptor GP38 in normal and malignant cell lines and tissues. / Weitman, Steven D; Lark, Richard H.; Coney, Leslie R.; Fort, Daniel W.; Frasca, Verna; Zurawski, Vincent R.; Kamen, Barton A.

In: Cancer Research, Vol. 52, No. 12, 15.06.1992, p. 3396-3401.

Research output: Contribution to journalArticle

Weitman, SD, Lark, RH, Coney, LR, Fort, DW, Frasca, V, Zurawski, VR & Kamen, BA 1992, 'Distribution of the folate receptor GP38 in normal and malignant cell lines and tissues', Cancer Research, vol. 52, no. 12, pp. 3396-3401.
Weitman SD, Lark RH, Coney LR, Fort DW, Frasca V, Zurawski VR et al. Distribution of the folate receptor GP38 in normal and malignant cell lines and tissues. Cancer Research. 1992 Jun 15;52(12):3396-3401.
Weitman, Steven D ; Lark, Richard H. ; Coney, Leslie R. ; Fort, Daniel W. ; Frasca, Verna ; Zurawski, Vincent R. ; Kamen, Barton A. / Distribution of the folate receptor GP38 in normal and malignant cell lines and tissues. In: Cancer Research. 1992 ; Vol. 52, No. 12. pp. 3396-3401.
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