Rat livers were cyclically perfused with 125I-asialofetuin, and after various time intervals, mitochondrial-lysosomal fractions were layered on colloidal silica gradients. We observed a time-dependent progression of trichloroacetic acid-precipitable radioactivity from the plasma membrane through a "buoyant" region, which contained lysosomal marker and other subcellular particles, to a dense region of relatively pure lysosomes. The buoyant peak of radioactivity represents an intermediate step in the transfer of asialofetuin from the plasma membrane to lysosomes since a) it appears prior to the release of acid-soluble degradation products and b) it can be dissociated from lysosomal markers.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Aug 31 1982|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology