Distribution of specific apolipoproteins determined by immunoblotting of baboon lipoproteins resolved by polyacrylamide gradient gel electrophoresis

David L. Rainwater, Candace M. Kammerer, Min Lee Cheng, Mary L. Sparks, John L. VandeBerg

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A method for the quantitative assessment of apolipoprotein distributions among baboon serum lipoproteins is described. The method combines the precise and reproducible separation of lipoproteins by polyacrylamide gradient gel electrophoresis with the specificity of immunoblotting. The method permits the measurement of distributions for any apolipoprotein for which there are antibodies available. Radioactive secondary antibodies are used to expose X-ray film, and distributions are determined by densitometry. Absorbance is linearly related to both antigen and antibody concentrations. The method is reproducible, with the mean coefficient of variation calculated to be 0.118, and has a high repeatability (r 2=0.97). The immunoblotting method can be employed to measure the fine details of lipoprotein phenotypes as they are influenced by genotype and environment.

Original languageEnglish (US)
Pages (from-to)143-158
Number of pages16
JournalBiochemical Genetics
Volume30
Issue number3-4
DOIs
StatePublished - Apr 1992
Externally publishedYes

Fingerprint

apolipoproteins
Papio
Apolipoproteins
polyacrylamide
immunoblotting
Electrophoresis
Immunoblotting
lipoproteins
gel electrophoresis
Lipoproteins
Polyacrylamide Gel Electrophoresis
electrokinesis
gel
Gels
Antibodies
antibody
X ray films
antibodies
densitometry
methodology

Keywords

  • apolipoproteins
  • gradient gel electrophoresis
  • immunoblotting
  • lipoproteins
  • primates

ASJC Scopus subject areas

  • Genetics
  • Biochemistry

Cite this

Distribution of specific apolipoproteins determined by immunoblotting of baboon lipoproteins resolved by polyacrylamide gradient gel electrophoresis. / Rainwater, David L.; Kammerer, Candace M.; Cheng, Min Lee; Sparks, Mary L.; VandeBerg, John L.

In: Biochemical Genetics, Vol. 30, No. 3-4, 04.1992, p. 143-158.

Research output: Contribution to journalArticle

Rainwater, David L. ; Kammerer, Candace M. ; Cheng, Min Lee ; Sparks, Mary L. ; VandeBerg, John L. / Distribution of specific apolipoproteins determined by immunoblotting of baboon lipoproteins resolved by polyacrylamide gradient gel electrophoresis. In: Biochemical Genetics. 1992 ; Vol. 30, No. 3-4. pp. 143-158.
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