Distribution of specific apolipoproteins determined by immunoblotting of baboon lipoproteins resolved by polyacrylamide gradient gel electrophoresis

David L. Rainwater; Candace M. Kammerer; Min Lee Cheng; Mary L. Sparks; John L. VandeBerg

doi:10.1007/BF02399705

Distribution of specific apolipoproteins determined by immunoblotting of baboon lipoproteins resolved by polyacrylamide gradient gel electrophoresis

David L. Rainwater, Candace M. Kammerer, Min Lee Cheng, Mary L. Sparks, John L. VandeBerg

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11 Scopus citations

Abstract

A method for the quantitative assessment of apolipoprotein distributions among baboon serum lipoproteins is described. The method combines the precise and reproducible separation of lipoproteins by polyacrylamide gradient gel electrophoresis with the specificity of immunoblotting. The method permits the measurement of distributions for any apolipoprotein for which there are antibodies available. Radioactive secondary antibodies are used to expose X-ray film, and distributions are determined by densitometry. Absorbance is linearly related to both antigen and antibody concentrations. The method is reproducible, with the mean coefficient of variation calculated to be 0.118, and has a high repeatability (r²=0.97). The immunoblotting method can be employed to measure the fine details of lipoprotein phenotypes as they are influenced by genotype and environment.

Original language	English (US)
Pages (from-to)	143-158
Number of pages	16
Journal	Biochemical Genetics
Volume	30
Issue number	3
DOIs	https://doi.org/10.1007/BF02399705
State	Published - Apr 1992

Keywords

apolipoproteins
gradient gel electrophoresis
immunoblotting
lipoproteins
primates

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Biochemistry
Molecular Biology
Genetics

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10.1007/BF02399705

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title = "Distribution of specific apolipoproteins determined by immunoblotting of baboon lipoproteins resolved by polyacrylamide gradient gel electrophoresis",

abstract = "A method for the quantitative assessment of apolipoprotein distributions among baboon serum lipoproteins is described. The method combines the precise and reproducible separation of lipoproteins by polyacrylamide gradient gel electrophoresis with the specificity of immunoblotting. The method permits the measurement of distributions for any apolipoprotein for which there are antibodies available. Radioactive secondary antibodies are used to expose X-ray film, and distributions are determined by densitometry. Absorbance is linearly related to both antigen and antibody concentrations. The method is reproducible, with the mean coefficient of variation calculated to be 0.118, and has a high repeatability (r2=0.97). The immunoblotting method can be employed to measure the fine details of lipoprotein phenotypes as they are influenced by genotype and environment.",

keywords = "apolipoproteins, gradient gel electrophoresis, immunoblotting, lipoproteins, primates",

author = "Rainwater, {David L.} and Kammerer, {Candace M.} and Cheng, {Min Lee} and Sparks, {Mary L.} and VandeBerg, {John L.}",

year = "1992",

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T1 - Distribution of specific apolipoproteins determined by immunoblotting of baboon lipoproteins resolved by polyacrylamide gradient gel electrophoresis

AU - Rainwater, David L.

AU - Kammerer, Candace M.

AU - Cheng, Min Lee

AU - Sparks, Mary L.

AU - VandeBerg, John L.

PY - 1992/4

Y1 - 1992/4

N2 - A method for the quantitative assessment of apolipoprotein distributions among baboon serum lipoproteins is described. The method combines the precise and reproducible separation of lipoproteins by polyacrylamide gradient gel electrophoresis with the specificity of immunoblotting. The method permits the measurement of distributions for any apolipoprotein for which there are antibodies available. Radioactive secondary antibodies are used to expose X-ray film, and distributions are determined by densitometry. Absorbance is linearly related to both antigen and antibody concentrations. The method is reproducible, with the mean coefficient of variation calculated to be 0.118, and has a high repeatability (r2=0.97). The immunoblotting method can be employed to measure the fine details of lipoprotein phenotypes as they are influenced by genotype and environment.

AB - A method for the quantitative assessment of apolipoprotein distributions among baboon serum lipoproteins is described. The method combines the precise and reproducible separation of lipoproteins by polyacrylamide gradient gel electrophoresis with the specificity of immunoblotting. The method permits the measurement of distributions for any apolipoprotein for which there are antibodies available. Radioactive secondary antibodies are used to expose X-ray film, and distributions are determined by densitometry. Absorbance is linearly related to both antigen and antibody concentrations. The method is reproducible, with the mean coefficient of variation calculated to be 0.118, and has a high repeatability (r2=0.97). The immunoblotting method can be employed to measure the fine details of lipoprotein phenotypes as they are influenced by genotype and environment.

KW - apolipoproteins

KW - gradient gel electrophoresis

KW - immunoblotting

KW - lipoproteins

KW - primates

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Distribution of specific apolipoproteins determined by immunoblotting of baboon lipoproteins resolved by polyacrylamide gradient gel electrophoresis

Abstract

Keywords

ASJC Scopus subject areas

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