Distinguishing benign from malignant pleural effusions by lectin immunocytochemistry

E. Rosen-Levin, J. R. Patil, C. W. Watson, J. Jagirdar

Research output: Contribution to journalArticle

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Abstract

Since distinguishing malignant from benign cells in pleural effusions can be difficult, with reactive mesothelial cells simulating adenocarcinoma cells, the binding patterns of a battery of lectins on cells in eight benign and eight malignant effusions were studied using the avidin-biotin peroxidase complex method. The following lectins were used: concanavalin A, Dolichos biflorus agglutinin, peanut agglutinin, Phaseolus vulgaris agglutinin, Ricinus communis germ agglutinin, soybean agglutinin, Ulex europeaus agglutinin (UEA) and wheat germ agglutinin. Several patterns of staining were seen with the lectins, but only UEA was helpful in distinguishing between benign and malignant effusions. Sixty percent of the adenocarcinomas stained with UEA, whereas none of the cells in the benign effusions did. These results imply that UEA positivity is indicative of carcinoma and can be useful in separating reactive or atypical mesothelial cells from adenocarcinoma cells.

Original languageEnglish (US)
Pages (from-to)499-504
Number of pages6
JournalActa Cytologica
Volume33
Issue number4
StatePublished - 1989
Externally publishedYes

Fingerprint

Malignant Pleural Effusion
Lectins
Agglutinins
Ulex
Immunohistochemistry
Adenocarcinoma
Peanut Agglutinin
Wheat Germ Agglutinins
Phaseolus
Avidin
Pleural Effusion
Biotin
Concanavalin A
Peroxidase
Staining and Labeling
Carcinoma

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy
  • Histology

Cite this

Rosen-Levin, E., Patil, J. R., Watson, C. W., & Jagirdar, J. (1989). Distinguishing benign from malignant pleural effusions by lectin immunocytochemistry. Acta Cytologica, 33(4), 499-504.

Distinguishing benign from malignant pleural effusions by lectin immunocytochemistry. / Rosen-Levin, E.; Patil, J. R.; Watson, C. W.; Jagirdar, J.

In: Acta Cytologica, Vol. 33, No. 4, 1989, p. 499-504.

Research output: Contribution to journalArticle

Rosen-Levin, E, Patil, JR, Watson, CW & Jagirdar, J 1989, 'Distinguishing benign from malignant pleural effusions by lectin immunocytochemistry', Acta Cytologica, vol. 33, no. 4, pp. 499-504.
Rosen-Levin E, Patil JR, Watson CW, Jagirdar J. Distinguishing benign from malignant pleural effusions by lectin immunocytochemistry. Acta Cytologica. 1989;33(4):499-504.
Rosen-Levin, E. ; Patil, J. R. ; Watson, C. W. ; Jagirdar, J. / Distinguishing benign from malignant pleural effusions by lectin immunocytochemistry. In: Acta Cytologica. 1989 ; Vol. 33, No. 4. pp. 499-504.
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