Distinct temporal genetic signatures of neurogenic and gliogenic cues in cortical stem cell cultures

Claire Sauvageot, Patricia L. Dahia, Ovidiu Lipan, John K. Park, Mi Sook Chang, John A. Alberta, Charles D. Stiles

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Cortical progenitor cells from rat embryos give rise to neurons or glia following exposure to platelet derived growth factor (PDGF) or ciliary neurotrophic factor (CNTF), respectively. Both growth factors impart their developmental cues quickly through a transcription-dependent mechanism. Do the alternate developmental responses to PDGF and CNTF reflect induction of qualitatively distinct genes? Alternatively, do the same genes respond to each growth factor, but with quantitatively distinct kinetics? Using differential library screening and custom cDNA microarrays we show that a common set of genes responds to either growth factor. However, quantitative differences in the onset and duration of gene induction equate to the expression of factor-specific gene signatures. Multitissue cluster analysis also reveals tissue-specific gene signatures that may play important roles in the developing brain.

Original languageEnglish (US)
Pages (from-to)121-133
Number of pages13
JournalJournal of Neurobiology
Volume62
Issue number1
DOIs
StatePublished - Jan 2005
Externally publishedYes

Keywords

  • Cortical stem cells
  • Differentiation
  • Expression analysis
  • Growth factors

ASJC Scopus subject areas

  • Neuroscience(all)
  • Cellular and Molecular Neuroscience

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