Distinct roles for CREB-binding protein and p300 in hematopoietic stem cell self-renewal

Vivienne I. Rebel; Andrew L. Kung; Elizabeth A. Tanner; Hong Yang; Roderick T. Bronson; David M. Livingston

doi:10.1073/pnas.232568499

Distinct roles for CREB-binding protein and p300 in hematopoietic stem cell self-renewal

Vivienne I. Rebel, Andrew L. Kung, Elizabeth A. Tanner, Hong Yang, Roderick T. Bronson, David M. Livingston

Greehey Children's Cancer Research Institute

Research output: Contribution to journal › Article › peer-review

167 Scopus citations

Abstract

Hematopoietic stem cells (HSC) are tightly regulated through, as yet, undefined mechanisms that balance self-renewal and differentiation. We have identified a role for the transcriptional coactivators CREB-binding protein (CBP) and p300 in such HSC fate decisions. A full dose of CBP, but not p300, is crucial for HSC self-renewal. Conversely, p300, but not CBP, is essential for proper hematopoietic differentiation. Furthermore, in chimeric mice, hematologic malignancies emerged from both CBP^-/- and p300^-/- cell populations. Thus, CBP and p300 play essential but distinct roles in maintaining normal hematopoiesis, and, in mice, both are required for preventing hematologic tumorigenesis.

Original language	English (US)
Pages (from-to)	14789-14794
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	99
Issue number	23
DOIs	https://doi.org/10.1073/pnas.232568499
State	Published - Nov 12 2002

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.232568499

Fingerprint Dive into the research topics of 'Distinct roles for CREB-binding protein and p300 in hematopoietic stem cell self-renewal'. Together they form a unique fingerprint.

Cite this

Distinct roles for CREB-binding protein and p300 in hematopoietic stem cell self-renewal. / Rebel, Vivienne I.; Kung, Andrew L.; Tanner, Elizabeth A.; Yang, Hong; Bronson, Roderick T.; Livingston, David M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 23, 12.11.2002, p. 14789-14794.

Research output: Contribution to journal › Article › peer-review

@article{5cbe808889a743d9982f65d32b2f6e7b,

title = "Distinct roles for CREB-binding protein and p300 in hematopoietic stem cell self-renewal",

abstract = "Hematopoietic stem cells (HSC) are tightly regulated through, as yet, undefined mechanisms that balance self-renewal and differentiation. We have identified a role for the transcriptional coactivators CREB-binding protein (CBP) and p300 in such HSC fate decisions. A full dose of CBP, but not p300, is crucial for HSC self-renewal. Conversely, p300, but not CBP, is essential for proper hematopoietic differentiation. Furthermore, in chimeric mice, hematologic malignancies emerged from both CBP-/- and p300-/- cell populations. Thus, CBP and p300 play essential but distinct roles in maintaining normal hematopoiesis, and, in mice, both are required for preventing hematologic tumorigenesis.",

author = "Rebel, {Vivienne I.} and Kung, {Andrew L.} and Tanner, {Elizabeth A.} and Hong Yang and Bronson, {Roderick T.} and Livingston, {David M.}",

year = "2002",

month = nov,

day = "12",

doi = "10.1073/pnas.232568499",

language = "English (US)",

volume = "99",

pages = "14789--14794",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "23",

}

TY - JOUR

T1 - Distinct roles for CREB-binding protein and p300 in hematopoietic stem cell self-renewal

AU - Rebel, Vivienne I.

AU - Kung, Andrew L.

AU - Tanner, Elizabeth A.

AU - Yang, Hong

AU - Bronson, Roderick T.

AU - Livingston, David M.

PY - 2002/11/12

Y1 - 2002/11/12

N2 - Hematopoietic stem cells (HSC) are tightly regulated through, as yet, undefined mechanisms that balance self-renewal and differentiation. We have identified a role for the transcriptional coactivators CREB-binding protein (CBP) and p300 in such HSC fate decisions. A full dose of CBP, but not p300, is crucial for HSC self-renewal. Conversely, p300, but not CBP, is essential for proper hematopoietic differentiation. Furthermore, in chimeric mice, hematologic malignancies emerged from both CBP-/- and p300-/- cell populations. Thus, CBP and p300 play essential but distinct roles in maintaining normal hematopoiesis, and, in mice, both are required for preventing hematologic tumorigenesis.

AB - Hematopoietic stem cells (HSC) are tightly regulated through, as yet, undefined mechanisms that balance self-renewal and differentiation. We have identified a role for the transcriptional coactivators CREB-binding protein (CBP) and p300 in such HSC fate decisions. A full dose of CBP, but not p300, is crucial for HSC self-renewal. Conversely, p300, but not CBP, is essential for proper hematopoietic differentiation. Furthermore, in chimeric mice, hematologic malignancies emerged from both CBP-/- and p300-/- cell populations. Thus, CBP and p300 play essential but distinct roles in maintaining normal hematopoiesis, and, in mice, both are required for preventing hematologic tumorigenesis.

UR - http://www.scopus.com/inward/record.url?scp=0037069424&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037069424&partnerID=8YFLogxK

U2 - 10.1073/pnas.232568499

DO - 10.1073/pnas.232568499

M3 - Article

C2 - 12397173

AN - SCOPUS:0037069424

VL - 99

SP - 14789

EP - 14794

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 23

ER -

Distinct roles for CREB-binding protein and p300 in hematopoietic stem cell self-renewal

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this