TY - JOUR
T1 - Distinct Pathways for snoRNA and mRNA Termination
AU - Kim, Minkyu
AU - Vasiljeva, Lidia
AU - Rando, Oliver J.
AU - Zhelkovsky, Alexander
AU - Moore, Claire
AU - Buratowski, Stephen
N1 - Funding Information:
We thank W. Keller, E. Steinmetz, D. Brow, J. Corden, G. Chanfreau, D. Ursic, and M. Culbertson for strains and plasmids; D. Brow, J. Corden, and D. Libri for communicating unpublished results; and J. Corden and D. Brow for helpful discussions. This research was supported by grants GM56663 to S.B. and GM68887 to C.M. from the US National Institutes of Health. L.V. is a Fellow of the Leukemia and Lymphoma Society. M.K. is supported by the Charles A. King Trust Postdoctoral Fellowship (Charles A. King Trust, Bank of America, Co-Trustee [Boston, Massachusetts]).
PY - 2006/12/8
Y1 - 2006/12/8
N2 - Transcription termination at mRNA genes is linked to polyadenylation. Cleavage at the poly(A) site generates an entry point for the Rat1/Xrn2 exonuclease, which degrades the downstream transcript to promote termination. Small nucleolar RNAs (snoRNAs) are also transcribed by RNA polymerase II but are not polyadenylated. Chromatin immunoprecipitation experiments show that polyadenylation factors and Rat1 localize to snoRNA genes, but mutations that disrupt poly(A) site cleavage or Rat1 activity do not lead to termination defects at these genes. Conversely, mutations of Nrd1, Sen1, and Ssu72 affect termination at snoRNAs but not at several mRNA genes. The exosome complex was required for 3′ trimming, but not termination, of snoRNAs. Both the mRNA and snoRNA pathways require Pcf11 but show differential effects of individual mutant alleles. These results suggest that in yeast the transcribing RNA polymerase II can choose between two distinct termination mechanisms but keeps both options available during elongation.
AB - Transcription termination at mRNA genes is linked to polyadenylation. Cleavage at the poly(A) site generates an entry point for the Rat1/Xrn2 exonuclease, which degrades the downstream transcript to promote termination. Small nucleolar RNAs (snoRNAs) are also transcribed by RNA polymerase II but are not polyadenylated. Chromatin immunoprecipitation experiments show that polyadenylation factors and Rat1 localize to snoRNA genes, but mutations that disrupt poly(A) site cleavage or Rat1 activity do not lead to termination defects at these genes. Conversely, mutations of Nrd1, Sen1, and Ssu72 affect termination at snoRNAs but not at several mRNA genes. The exosome complex was required for 3′ trimming, but not termination, of snoRNAs. Both the mRNA and snoRNA pathways require Pcf11 but show differential effects of individual mutant alleles. These results suggest that in yeast the transcribing RNA polymerase II can choose between two distinct termination mechanisms but keeps both options available during elongation.
KW - DNA
KW - RNA
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U2 - 10.1016/j.molcel.2006.11.011
DO - 10.1016/j.molcel.2006.11.011
M3 - Article
C2 - 17157255
AN - SCOPUS:33751506478
SN - 1097-2765
VL - 24
SP - 723
EP - 734
JO - Molecular Cell
JF - Molecular Cell
IS - 5
ER -