Distinct mechanisms of splicing regulation in vivo by the drosophila protein sex-lethal

Begoña Granadino, Luiz O.F. Penalva, Michael R. Green, Juán Valcárcel, Lucas Sánchez

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

The protein Sex-lethal (SXL) controls pre-mRNA splicing of two genes involved in Drosophila sex determination: transformer (tra) and the Sxl gene itself. Previous in vitro results indicated that SXL antagonizes the general splicing factor U2AF65 to regulate splicing of tra. In this report, we have used transgenic flies expressing chimeric proteins between SXL and the effector domain of U2AF65 to study the mechanisms of splicing regulation by SXL in vivo. Conferring U2AF activity to SXL relieves its inhibitory activity on tra splicing but not on Sxl splicing. Therefore, antagonizing U2AF65 can explain tra splicing regulation both in vitro and in vivo, but this mechanism cannot explain splicing regulation of Sxl pre-mRNA. These results are a direct proof that Sxl, the master regulatory gene in sex determination, has multiple and separable activities in the regulation of pre-mRNA splicing.

Original languageEnglish (US)
Pages (from-to)7343-7348
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number14
DOIs
StatePublished - Jul 8 1997

Keywords

  • Transformer
  • U2AF-Sx1 chimeric transgenes

ASJC Scopus subject areas

  • General

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