Distinct Functional and Temporal Requirements for Zebrafish Hdac1 during Neural Crest-Derived Craniofacial and Peripheral Neuron Development

Myron S Ignatius, Arife Unal Eroglu, Smitha Malireddy, Glen Gallagher, Roopa M. Nambiar, Paul D. Henion

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The regulation of gene expression is accomplished by both genetic and epigenetic means and is required for the precise control of the development of the neural crest. In hdac1b382 mutants, craniofacial cartilage development is defective in two distinct ways. First, fewer hoxb3a, dlx2 and dlx3-expressing posterior branchial arch precursors are specified and many of those that are consequently undergo apoptosis. Second, in contrast, normal numbers of progenitors are present in the anterior mandibular and hyoid arches, but chondrocyte precursors fail to terminally differentiate. In the peripheral nervous system, there is a disruption of enteric, DRG and sympathetic neuron differentiation in hdac1b382 mutants compared to wildtype embryos. Specifically, enteric and DRG-precursors differentiate into neurons in the anterior gut and trunk respectively, while enteric and DRG neurons are rarely present in the posterior gut and tail. Sympathetic neuron precursors are specified in hdac1b382 mutants and they undergo generic neuronal differentiation but fail to undergo noradrenergic differentiation. Using the HDAC inhibitor TSA, we isolated enzyme activity and temporal requirements for HDAC function that reproduce hdac1b382 defects in craniofacial and sympathetic neuron development. Our study reveals distinct functional and temporal requirements for zebrafish hdac1 during neural crest-derived craniofacial and peripheral neuron development.

Original languageEnglish (US)
Article numbere63218
JournalPLoS One
Volume8
Issue number5
DOIs
StatePublished - May 7 2013
Externally publishedYes

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neural crest
Neural Crest
Zebrafish
Danio rerio
Neurons
neurons
Diagnosis-Related Groups
Arches
mutants
digestive system
Branchial Region
peripheral nervous system
Histone Deacetylase Inhibitors
chondrocytes
Peripheral Nervous System
gene expression regulation
Cartilage
Gene Expression Regulation
Enzyme activity
Neurology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Distinct Functional and Temporal Requirements for Zebrafish Hdac1 during Neural Crest-Derived Craniofacial and Peripheral Neuron Development. / Ignatius, Myron S; Unal Eroglu, Arife; Malireddy, Smitha; Gallagher, Glen; Nambiar, Roopa M.; Henion, Paul D.

In: PLoS One, Vol. 8, No. 5, e63218, 07.05.2013.

Research output: Contribution to journalArticle

Ignatius, Myron S ; Unal Eroglu, Arife ; Malireddy, Smitha ; Gallagher, Glen ; Nambiar, Roopa M. ; Henion, Paul D. / Distinct Functional and Temporal Requirements for Zebrafish Hdac1 during Neural Crest-Derived Craniofacial and Peripheral Neuron Development. In: PLoS One. 2013 ; Vol. 8, No. 5.
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