Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling

Yong Ge, Mojgan Zadeh, Mansour Mohamadzadeh

Research output: Contribution to journalArticlepeer-review

Abstract

Intestinal epithelium is composed of several cell types, which can be dissociated but difficult to maintain high cell viability due to anoikis. Herein, we describe a step-by-step protocol for the isolation of highly viable intestinal epithelial cells using ethylenediaminetetraacetate acid and TrypLE Express, which can subsequently be employed for multi-omic analyses, including single-cell RNA sequencing. For complete details on the use and execution of this protocol, please refer to Ge et al. (2022).1

Original languageEnglish (US)
Article number101936
JournalSTAR Protocols
Volume4
Issue number1
DOIs
StatePublished - Mar 17 2023

Keywords

  • Cell isolation
  • Flow Cytometry/Mass Cytometry
  • Sequencing
  • Single Cell

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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