Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling

Yong Ge; Mojgan Zadeh; Mansour Mohamadzadeh

doi:10.1016/j.xpro.2022.101936

Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling

Yong Ge, Mojgan Zadeh, Mansour Mohamadzadeh

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Intestinal epithelium is composed of several cell types, which can be dissociated but difficult to maintain high cell viability due to anoikis. Herein, we describe a step-by-step protocol for the isolation of highly viable intestinal epithelial cells using ethylenediaminetetraacetate acid and TrypLE Express, which can subsequently be employed for multi-omic analyses, including single-cell RNA sequencing. For complete details on the use and execution of this protocol, please refer to Ge et al. (2022).¹

Original language	English (US)
Article number	101936
Journal	STAR Protocols
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2022.101936
State	Published - Mar 17 2023

Keywords

Cell isolation
Flow Cytometry/Mass Cytometry
Sequencing
Single Cell

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.1016/j.xpro.2022.101936

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KW - Single Cell

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Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling

Abstract

Keywords

ASJC Scopus subject areas

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