Dishevelled, a Wnt signalling component, is involved in mitotic progression in cooperation with Plk1

Koji Kikuchi, Yohei Niikura, Katsumi Kitagawa, Akira Kikuchi

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Wnt signalling is known to promote G1/S progression through the stimulation of gene expression, but whether this signalling regulates mitotic progression is not clear. Here, the function of dishevelled 2 (Dvl2), which transmits the Wnt signal, in mitosis was examined. Dvl2 localized to the spindles and spindle poles during mitosis. When cells were treated with nocodazole, Dvl2 was observed at the kinetochores (KTs). Dvl2 bound to and was phosphorylated at Thr206 by a mitotic kinase, Polo-like kinase 1 (Plk1), and this phosphorylation was required for spindle orientation and stable microtubule (MT)-KT attachment. Dvl2 was also found to be involved in the activation of a spindle assembly checkpoint (SAC) kinase, Mps1, and the recruitment of other SAC components, Bub1 and BubR1, to the KTs. However, the phosphorylation of Dvl2 by Plk1 was dispensable for SAC. Furthermore, Wnt receptors were involved in spindle orientation, but not in MT-KT attachment or SAC. These results suggested that Dvl2 is involved in mitotic progression by regulating the dynamics of MT plus-ends and the SAC in Plk1-dependent and-independent manners.

Original languageEnglish (US)
Pages (from-to)3470-3483
Number of pages14
JournalEMBO Journal
Volume29
Issue number20
DOIs
StatePublished - Oct 1 2010
Externally publishedYes

Fingerprint

M Phase Cell Cycle Checkpoints
Kinetochores
Microtubules
Phosphorylation
Mitosis
Phosphotransferases
Wnt Receptors
Spindle Poles
Nocodazole
Gene expression
Poles
Chemical activation
polo-like kinase 1
Gene Expression

Keywords

  • dishevelled
  • microtubule plus end
  • mitosis
  • Plk1
  • Wnt signalling

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Dishevelled, a Wnt signalling component, is involved in mitotic progression in cooperation with Plk1. / Kikuchi, Koji; Niikura, Yohei; Kitagawa, Katsumi; Kikuchi, Akira.

In: EMBO Journal, Vol. 29, No. 20, 01.10.2010, p. 3470-3483.

Research output: Contribution to journalArticle

Kikuchi, Koji ; Niikura, Yohei ; Kitagawa, Katsumi ; Kikuchi, Akira. / Dishevelled, a Wnt signalling component, is involved in mitotic progression in cooperation with Plk1. In: EMBO Journal. 2010 ; Vol. 29, No. 20. pp. 3470-3483.
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