Discovery of piperlongumine as a potential novel lead for the development of senolytic agents

Yingying Wang, Jianhui Chang, Xingui Liu, Xuan Zhang, Suping Zhang, Xin Zhang, Daohong Zhou, Guangrong Zheng

Research output: Contribution to journalArticlepeer-review

185 Scopus citations

Abstract

Accumulating evidence indicates that senescent cells play an important role in many age-associated diseases. The pharmacological depletion of senescent cells (SCs) with a "senolytic agent", a small molecule that selectively kills SCs, is a potential novel therapeutic approach for these diseases. Recently, we discovered ABT- 263, a potent and highly selective senolytic agent, by screening a library of rationally-selected compounds. With this screening approach, we also identified a second senolytic agent called piperlongumine (PL). PL is a natural product that is reported to have many pharmacological effects, including anti-tumor activity. We show here that PL preferentially killed senescent human WI-38 fibroblasts when senescence was induced by ionizing radiation, replicative exhaustion, or ectopic expression of the oncogene Ras. PL killed SCs by inducing apoptosis, and this process did not require the induction of reactive oxygen species. In addition, we found that PL synergistically killed SCs in combination with ABT-263, and initial structural modifications to PL identified analogs with improved potency and/or selectivity in inducing SC death. Overall, our studies demonstrate that PL is a novel lead for developing senolytic agents.

Original languageEnglish (US)
Pages (from-to)2915-2926
Number of pages12
JournalAging
Volume8
Issue number11
DOIs
StatePublished - 2016
Externally publishedYes

Keywords

  • ABT-263
  • Aging
  • Piperlongumine
  • Reactive oxygen species
  • Senescent cells
  • Senolytic agents
  • Synergistic effect

ASJC Scopus subject areas

  • Aging
  • Cell Biology

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