Discovery of a Cryptic Intermediate in Late Steps of Mithramycin Biosynthesis

Ryan Wheeler, Xia Yu, Caixia Hou, Prithiba Mitra, Jhong Min Chen, Frank Herkules, Dmitri N. Ivanov, Oleg V. Tsodikov, Jürgen Rohr

Research output: Contribution to journalArticle

Abstract

MtmOIV and MtmW catalyze the final two reactions in the mithramycin (MTM) biosynthetic pathway, the Baeyer–Villiger opening of the fourth ring of premithramycin B (PMB), creating the C3 pentyl side chain, strictly followed by reduction of the distal keto group on the new side chain. Unexpectedly this results in a C2 stereoisomer of mithramycin, iso-mithramycin (iso-MTM). Iso-MTM undergoes a non-enzymatic isomerization to MTM catalyzed by Mg2+ ions. Crystal structures of MtmW and its complexes with co-substrate NADPH and PEG, suggest a catalytic mechanism of MtmW. The structures also show that a tetrameric assembly of this enzyme strikingly resembles the ring-shaped β subunit of a vertebrate ion channel. We show that MtmW and MtmOIV form a complex in the presence of PMB and NADPH, presumably to hand over the unstable MtmOIV product to MtmW, yielding iso-MTM, as a potential self-resistance mechanism against MTM toxicity.

Original languageEnglish (US)
Pages (from-to)826-832
Number of pages7
JournalAngewandte Chemie - International Edition
Volume59
Issue number2
DOIs
StatePublished - Jan 7 2020

Fingerprint

Plicamycin
Biosynthesis
Ions
Isomerization
Polyethylene glycols
Toxicity
Enzymes
Crystal structure
NADP
Substrates
Stereoisomerism
Ion Channels

Keywords

  • biocatalysis
  • biosynthesis
  • isomerization
  • natural products
  • protein structures

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

Wheeler, R., Yu, X., Hou, C., Mitra, P., Chen, J. M., Herkules, F., ... Rohr, J. (2020). Discovery of a Cryptic Intermediate in Late Steps of Mithramycin Biosynthesis. Angewandte Chemie - International Edition, 59(2), 826-832. https://doi.org/10.1002/anie.201910241

Discovery of a Cryptic Intermediate in Late Steps of Mithramycin Biosynthesis. / Wheeler, Ryan; Yu, Xia; Hou, Caixia; Mitra, Prithiba; Chen, Jhong Min; Herkules, Frank; Ivanov, Dmitri N.; Tsodikov, Oleg V.; Rohr, Jürgen.

In: Angewandte Chemie - International Edition, Vol. 59, No. 2, 07.01.2020, p. 826-832.

Research output: Contribution to journalArticle

Wheeler, R, Yu, X, Hou, C, Mitra, P, Chen, JM, Herkules, F, Ivanov, DN, Tsodikov, OV & Rohr, J 2020, 'Discovery of a Cryptic Intermediate in Late Steps of Mithramycin Biosynthesis', Angewandte Chemie - International Edition, vol. 59, no. 2, pp. 826-832. https://doi.org/10.1002/anie.201910241
Wheeler, Ryan ; Yu, Xia ; Hou, Caixia ; Mitra, Prithiba ; Chen, Jhong Min ; Herkules, Frank ; Ivanov, Dmitri N. ; Tsodikov, Oleg V. ; Rohr, Jürgen. / Discovery of a Cryptic Intermediate in Late Steps of Mithramycin Biosynthesis. In: Angewandte Chemie - International Edition. 2020 ; Vol. 59, No. 2. pp. 826-832.
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